Molecular farming in plants is a relatively young subject of sciences. As plants can offer an inexpensive and convenient platform for the large-scale production of recombinant proteins with various functions, the driven force from the giant market for recombinant protein pharmaceuticals and industrial enzymes makes this subject grow and advance very quickly. To summarize recent advances, current challenges and future directions in molecular farming, international authorities were invited to write this book for researchers, teachers and students who are interested in this subject. This book, with the focus on the most advanced cutting-edge breakthroughs, covers all the essential aspects of the field of molecular farming in plants: from expression technologies to downstream processing, from products to safety issues, and from current advances and holdups to future developments.
Table of Content
Preface.- Contributors.- 1. Molecular Farming in Plants: an Overview; Shengwu Ma, Aiming Wang.- 2. Production of Foreign Proteins in Transgenic Plants; Shengwu Ma.- 3. Molecular Farming Using Bioreactor-based Plant Cell Suspension Cultures for Recombinant Protein Production; Ting-Kuo Huang, Karen A. Mc Donald.- 4. Chloroplast-derived Therapeutic and Prophylactic Vaccines; James S. New, Donevan Westerveld, Henry Daniell.- 5. Seed Expression Systems for Molecular Farming; Allison R. Kermode.- 6. Algae: an Alternative to the Higher Plant System in Gene Farming; Christoph Griesbeck, Anna Kirchmayr.- 7. The Production of Vaccines and Therapeutic Antibodies in Plants; Richard M. Twyman, Stefan Schillberg, Rainer Fischer.- 8. Production of Industrial Proteins in Plants; Elizabeth E. Hood, Deborah Vicuna Requesens.- 9. Transient Expression Using Agro-infiltration and Its Applications in Molecular Farming; Rima Menassa, Adil Ahmad, Jussi Joensuu.- 10. Plant Virus-mediated Expression in Molecular Farming; Aiming Wang.- 11. Downstream Processing of Transgenic Plant Systems: Protein Recovery and Purification Strategies; Lisa R. Wilken, Zivko L. Nikolov.- 12. Biosafety of Molecular Farming in Genetically Modified Plants; Didier Breyer et al.- Index.
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