Charles Oluwaseun Adetunji & Chukwuebuka Egbuna 
Handbook of Agricultural Biotechnology, Volume 5 [PDF ebook] 
Nanobiofertilizers

الدعم

This book details recent advances in the applications of nanobiofertilizers as a substitute for synthetic fertilizers in boosting food production.

With the steady rise of the world’s population, there is a need to increase the production of safe and nutritious food. The constant loss of arable land, as a result of various anthropogenic activities from human action, has become a threat to global biodiversity and ecosystems. Additionally, the issue of climate change has imposed many obstacles to increasing agricultural productivity, especially from biotic and abiotic stressors and temperature-limited environments, such as in high altitudes or seasonally hot regions. Because of these factors, there is a need to adopt sustainable and modern technologies that can boost and improve the rate of food production.

One of the cheapest means of enhancing sustainable food production is to explore natural and unlimited beneficial microorganisms, particularly those that can increase the level of soil fertility, improve crop production and health, improve tolerance to stress, support nutrient uptake and availability, and boost natural biodiversity. The synergetic effect of nanotechnology and beneficial microorganisms for the effective bio-fabrication of nanobiofertilizers, is a sustainable solution for producing pesticide-free food. This book provides a deep insight into microbial diversity, recent techniques used for the isolation, screening, and characterization of beneficial microorganisms with eco-friendly attributes, used for bioengineering of nanobiofertilizers, as well as the application of proteomics, metabolomics, genomics, and bioinformatics. The book also covers commercialization, patents, and the business and socio-economic aspects of nanobiofertilizers, as well as the role of policymakers, stakeholders, and government agencies in the translation of nanobioferilizer research into policy.

Audience

The book is a useful resource for a diverse audience, including industrialists, food industry professionals, agriculturists, agricultural microbiologists, plant pathologists, botanists, microbiologists, biotechnologists, nanotechnologists, microbial biotechnologists, farmers, policymakers, and extension workers.

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قائمة المحتويات

Preface xxiii

1 Application of Nanobiofertilization for Bioremediation and Ecorestoration of Polluted Soil/Farmland 1
Oluwafemi Adebayo Oyewole, Konjerimam Ishaku Chimbekujwo, Margaret Oniha, Isibor Patrick Omoregie, Opeyemi Isaac Ayanda, Charles Oluwaseun Adetunji and John Tsado Mathew

1.1 Introduction 2

1.2 Nanoparticles 3

1.3 Nanobiofertilization in Bioremediation 10

1.4 Application of Nanobiofertilization in Bioremediation 15

1.5 Environmental Distress 21

1.6 Conclusion 22

References 22

2 Influence of Nanobiofertilizer on Plant Yield and Growth 37
Samuel Adeniyi Oyegbade, Andrew Aladele Kolawole, Jerry Gbotemi Oni, Alhassan Muhammad Alhassan, Oluwafemi Adebayo Oyewole, Margaret Oniha and Charles Oluwaseun Adetunji

2.1 Introduction 38

Nanobiofertilizer 39

Composition of Nanobiofertilizer 40

Mechanisms of Nutrient Delivery and Enhanced Bioavailability 42

Seed Priming (Nanopriming) 42

Soil-Based Application 43

Nanobiofertilizer Plant System Interaction 43

Impact of Nanobiofertilizer on Plant Biomass 44

Factors Contributing to Enhanced Plant Growth 45

Comparison of Biomass Enhancement with Traditional Fertilizers 47

Nanobiofertilizer-Induced Biomass and Chlorophyll Content Enhancement 49

Crop-Specific Responses to Nanobiofertilizers 49

Case Studies Highlighting Positive Outcomes on Various Crops 50

Environmental and Economic Considerations on the Use of Nanobiofertilizers 52

Comparison of Nanobiofertilizers with Traditional Fertilizers in Terms of Cost and Effectiveness 53

Potential Long-Term Benefits for Soil Health and Ecosystem 54

Addressing Concerns Related to Nanoparticle Toxicity and Accumulation 55

Need for Standardized Testing Protocols and Safety Assessments 56

Exploration of Innovative Nanobiofertilizer Formulations and Delivery Methods 57

Strategies for Incorporating Nanobiofertilizers into Existing Farming Systems 57

Synergistic Effects of Combining Nanobiofertilizers with Other Sustainable Practices 58

Practical Considerations for Large-Scale Implementation 58

Potential to Revolutionize Agriculture and Contribute to Food Security 59

Call to Action for Continued Research, Development, and Adoption of Nanobiofertilizers 60

Conclusion 60

References 61

3 Effect of Bionanofertilizer on Proximate Composition of Crops 71
Oluwadurotimi Samuel Aworunse, Fadekemi Akinhanmi, Ogochukwu Onwaeze, Alhassan Muhammad Alhassan, Oluwafemi Adebayo Oyewole, Charles Oluwaseun Adetunji and John Tsado Mathew

3.1 Introduction 72

3.2 Biological Synthesis of Nanofertilizers 73

3.3 Composition of Bionanofertilizers 79

3.4 Properties of Bionanofertilizers 82

3.5 Effect of Bionanofertilizers of Proximate Parameters of Crops 84

3.6 Conclusion and Future Direction 87

References 88

4 The Role of Policy Maker, Relevant Stakeholders and Government Agency in Translating Nanobiofertilizer Research into Policy 99
Alhasssan Muhammed Alhassan, Ruth Ebunoluwa Bodunrinde, Oluwafemi Adebayo Oyewole, Charles Oluwaseun Adetunji and John Tsado Mathew

4.1 Introduction 100

4.2 Views of the Dangers of Nanotechnology and Confidence in Stakeholders 101

4.3 Policy Making Process 102

4.4 Benefits of Agencies in the Nanoproduction of Fertilizer 102

4.5 Relevant Stakeholders in the Implementation of Policy 102

4.6 Report of Nanobiofertilizers Worldwide 103

4.7 Government Agencies and Their Impact 103

4.8 Translating Research into Policy 104

4.9 Global Safety and Legal Framework for Agricultural Goods Based on Nanotechnology 107

4.10 Future Initiatives and Studies to Support the Development of Nanobiofertilizers 108

Conclusion 111

References 112

5 Structural Elucidation, Detection, and Characterization of Essential Nutrients Necessary for Soil Fertilization 117
John Tsado Mathew, Abel Inobeme, Charles Oluwaseun Adetunji, Yakubu Azeh, Abdulfatai Aideye Otori, Elijah Yanda Shaba, Musah Monday, Musa Safiyanu Tanko, Ezekiel Tanko, Amos Mamman, Jibrin Noah Akoji and Oluwafemi Adebayo Oyewole

5.1 Introduction 118

5.2 Nitrogen (N) in Soil Fertilization 123

5.3 Phosphorus (P) in Soil Fertilization 127

5.4 Potassium (K) in Soil Fertilization 131

5.5 Optimization of Nutrient Management Strategies 135

Conclusion 139

References 140

6 Effect of Nanobiofertilizer on Phytochemicals 149
John Tsado Mathew, Abel Inobeme, Charles Oluwaseun Adetunji, Yakubu Azeh, Abdulfatai Aideye Otori, Musah Monday, Elijah Yanda Shaba, Badeggi Muhammad Umar, Amos Mamman, Jemkur Maurice and Oluwafemi Adebayo Oyewole

6.1 General Overview on Nanobiofertilizer 150

6.2 Constituents of Nanobiofertilizer 151

6.3 Importance of Phytochemicals in Plant Growth and Human Health 158

6.4 Mechanisms of Nanobiofertilizer on Phytochemicals 160

6.5 Recent Studies on Effect of Nanobiofertilizer on Phytochemicals 163

6.6 Conclusion and Future Trends on Nanobiofertilizer on Phytochemicals 167

References 168

7 Characterization of Nanoparticles Used as Nanobiofertilizers 175
John Tsado Mathew, Charles Oluwaseun Adetunji, Abel Inobeme, Yakubu Azeh, Musah Monday, Etsuyankpa Muhammad Bini, Abdulfatai Aideye Otori, Elijah Yanda Shaba, Muhammad Aishetu Ibrahim, Musa Safiyanu Tanko, Amos Mamman and Oluwafemi Adebayo Oyewole

7.1 Introduction 176

7.2 Some Spectroscopic Characterization Technique for Nanomaterials 180

7.3 Characterization of Nanobiofertilizer Through Chemical and Biological Synthesis 187

7.4 Application of Nanobiofertilizer 189

7.5 Environmental Impact Assessment 191

7.6 Future Perspectives and Challenges 192

7.7 Conclusion 194

References 194

8 Toxicological Effects of Nanobiofertilizer on Water Body, Water Quality, Lower Plants, Zooplanktons, and Beneficial Microorganisms 201
Ezugwu Basil Utazi, Oluwafemi Adebayo Oyewole, Japhet Gaius Yakubu, Tsado Priscilla Yetu, Isibor Patrick Omoregie, Charles Oluwaseun Adetunji, John Tsado Mathew, Victory Igiku, Eniola K. I. T. and Mohammed Bello Yerima

8.1 Introduction 202

8.2 Effects of Nanofertilizer on Soil Microbial Community 203

8.3 Nanofertilizers Versus Biofertilizers: Dissimilarity in Synthesis 205

8.4 Nanobiofertilizer 206

8.5 Conclusion 217

References 219

9 Various Techniques Used in the Application of Nanobiofertilizers on Crops After Synthesis 233
Victory Igiku, Charles Oluwaseun Adetunji, Oluwafemi Adebayo Oyewole, Fadekemi O. Akinhanmi, Simon Sunday Ameh, Eniola K. I. T. and Mohammed Bello Yerima

9.1 Introduction 234

9.2 Synthesis of Nanoparticles 235

9.3 Synthesis of Nanobiofertilizers 239

9.4 Methods Used in the Application of Nanobiofertilizers on Crops 240

9.5 Conclusion 244

References 244

10 Non-Target Effect, Environmental Impact, and Assessment of Nanobiofertilizer 253
Fadekemi O. Akinhanmi, Oluwadurotimi S. Aworunse, Oluwafemi Adebayo Oyewole, Charles Oluwaseun Adetunji, John Tsado Mathew, Victory Igiku, Eniola K. I. T. and Mohammed Bello Yerima

10.1 Introduction 254

10.2 Environmental Impact of Nanobiofertilizer on Soil Properties 256

10.3 Non-Targeted Effects on Soil Properties 257

10.4 Recommendation and Conclusion 264

References 266

11 Reported Genes Regulating the Biological Activities in Microorganisms Used in the Formation of Nanobiofertilizers 271
Victory Igiku, Charles Oluwaseun Adetunji, Oluwafemi Adebayo Oyewole, Eniola K. I. T. and Mohammed Bello Yerima

11.1 Introduction 272

11.2 Synthesis of Nanoparticles 273

11.3 Genes Regulating the Biological Activities in Plant Growth-Promoting Rhizobacteria 277

11.4 Conclusion 283

References 284

12 Relevance of Molecular Genetics and Synthetic Biology Involved in the Characterization of Microorganisms Used in Nanofertilizer Research 291
Simon Sunday Ameh, Daniel Gana, Mordecai Gana, Japheth Gaius Yakubu, Amarachi Rosemary Osi, Oluwafemi Adebayo Oyewole, Charles Oluwaseun Adetunji, John Tsado Mathew, Victory Igiku, Eniola K. I. T. and Mohammed Bello Yerima

12.1 Introduction 292

12.2 Molecular Genetics and Synthetic Biology 293

12.3 Nanotechnology 299

12.4 Constituents of Nanobiofertilizer 300

12.5 Formulation of Nanobiofertilizer 304

12.6 Microorganisms Employed in Nanobiofertilizer 304

12.7 Genetic Techniques Used to Study Microorganisms in Nanobiofertilizers 305

12.8 Conclusion 306

12.9 Recommendation 307

References 308

13 Techniques Used for Screening and Detection of Beneficial Microorganisms Used for Production of Nanobiofertilizers 315
Idris Abdullahi Dabban, Aisha Bisola Bello, Adioha Amarachi, Adejoh Suleiman Ocholi., Dewu Mansur Muhammad, Oko Joseph Odey, Oluwafemi Adebayo Oyewole, Charles Oluwaseun Adetunji, Eniola Kehinde Imisiagbaraolorun Temitope and Mohammed Bello Yerima

13.1 Introduction 316

13.2 Beneficial Microorganisms Used for Production of Nanobiofertilizers: Sources and Characteristic Traits 320

13.3 Techniques Used for Screening and Detection of Nanobiofertilizer-Producing Microbes 327

13.4 Molecular Techniques Used for Detection of Beneficial Microbes for Nanobiofertilizer Production 333

Future Perspectives 337

References 338

14 Toxicology and Adverse Effects of Chemical Fertilizer and Nanobiofertilizer Pollution of the Environment; Bioaccumulation, Greenhouse Effects, and Global Warming 347
Oluwafemi Adebayo Oyewole, Japhet Gaius Yakubu, Sani Rabiu Aishat, Iseghohi Frances, Tsado Priscilla Yetu, Opeyemi Isaac Ayanda, Charles Oluwaseun Adetunji, Eniola K. I. T. and Mohammed Bello Yerima

14.1 Introduction 348

14.2 Chemical Fertilizers 350

14.3 Environmental Pollution Caused by Use of Chemical and Nanofertilizers 357

14.4 Toxicological Effects of Fertilizers 359

14.5 Conclusion 370

14.6 Future Perspective 371

References 371

15 Effect of Climate Changes, Weather and Other Environmental Factors Whenever Nanobiofertilizer is Applied for Crop Improvement 377
Temitope Ayinde Oluwaseyi, Abah Daniel Okochegbe, Oluwafemi Adebayo Oyewole, Samuel Adeniyi Oyegbade, Enitan Emmanuella Lawal, Charles Oluwaseun Adetunji, Eniola K. I. T. and Mohammed Bello Yerima

15.1 Introduction 378

15.2 Nanobiofertilizers 380

15.3 Nanobiofertilizer: Pertinence to Trim Improvement 381

15.4 Nanobiofertilizer: Job in Crop Improvement 381

15.5 Nanobiofertilizer: Capability in Yield Well-Being 382

15.6 Progress Made with Nanobiofertilizers 382

15.7 Crop Improvement Outcomes Using Nanobiofertilizers 383

15.8 Environmental Factors and the Interaction of Nanobiofertilizers 383

15.9 Climate 386

15.10 Synergies and Challenges: Nanobiofertilizers in a Changing Climate 387

15.11 Weather Patterns and Nano Biofertilizer Performance 387

15.12 Strategies to Overcome Impact of Climate Changes 389

15.13 Conclusion 390

References 391

16 Effect of Nanobiofertilizer on Essential Amino Acid Constituents 395
Margaret Ikhiwili Oniha, Konjerimam Ishaku Chimbekujwo, Aisha Bisola Bello, Mordecai Gana, Oluwafemi Adebayo Oyewole, Charles Oluwaseun Adetunji, Kehinde Imisiagbaraolorun Temitope Eniola and Mohammed Bello Yerima

16.1 Introduction 396

16.2 Biofertilizers 397

16.3 Amino Acids 399

16.4 Nanofertilizers: Effect of Nanobiofertilizers on Amino Acids in Plant Growth 401

Conclusion 406

References 407

17 Relevance of Nanotechnology in Agriculture 413
Bello Oluwakemi Adetutu, Adamu Binta Buba, Fadekemi Akinhanmi, Mordecai Gana, Ezugwu Basil Utazi, Oluwafemi Adebayo Oyewole, Charles Oluwaseun Adetunji, Eniola K. I. T. and Mohammed Bello Yerima

17.1 Introduction 414

17.2 Nanotechnology’s Significance Across Industries 417

17.3 Nanotechnology in Agriculture 421

17.4 Nanotechnology in Crop Production 422

17.5 Nano-Based Crop Protection 435

17.6 Precision Farming and Sensors 440

17.7 Enhanced Nutrient Management: Utilizing Nanotechnology for Controlled Nutrient Release and Minimized Runoff 443

17.8 Challenges and Ethical Considerations in the Use of Nanomaterials in Agriculture 445

17.9 Future Directions and Research Opportunities in Nanotechnology for Agriculture 447

17.10 Conclusion 449

References 450

18 Recent Advancement Toward the Application of Proteomics, Metabolomics, Genomics and Bioinformatics for the Improvement of Nanofertilizer Research 463
Oluwafemi Adebayo Oyewole, Clement Shina Olusanya, Japhet Gaius Yakubu, Oluwadurotimi Samuel Aworunse, Ezugwu, Basil Utazi, Charles Oluwaseun Adetunji, Eniola K. I. T. and Mohammed Bello Yerima

18.1 Nanobiofertilizer 464

18.2 Constituents of Nanobiofertilizer 466

18.3 Nanobiofertilizer Preparation 476

18.4 Nanobiofertilizer and Soil Fertility 477

18.5 Response of Plant to Nanobiofertilizer 477

18.6 Setback and Impact of Nanofertilizer in the Framework of Sustainable Agriculture 478

18.7 Biotechnology Tools Used in Agriculture 480

18.8 Present Status of Nanobiofertilizer Research and Application 485

18.9 Application of Biotechnology Tools for Nanobiofertilizer Improvement 486

18.10 Conclusion 487

References 487

19 Isolation Techniques Used for Molecular Characterization of Beneficial Microorganisms: Cultural, Biochemical and Molecular Characterization 491
Idris Abdullahi Dabban, Muazu Ahmad, Sherifat Ozavize Enejiyon, Ahmad Nana Hauwau, Mustapha Gani, Oluwafemi Adebayo Oyewole and Charles Oluwaseun Adetunji

19.1 Introduction 492

19.2 Techniques for Isolation and Characterization of Bacteria and Actinomycetes: Cultural, Biochemical, and Molecular (16sr RNA) 496

19.3 Techniques for Isolation and Characterization of Fungi 509

19.4 Methods for Isolation and Characterization of Algae 523

19.5 Conclusion and Future Perspective 530

References 531

20 Nanoparticle: Characterization and Their Potential Role as Revolutionary Nanobiofertilizer in Agriculture 547
Nisha Sogan, Smriti Kala and Alka Rani

20.1 Introduction 547

20.2 Silver Nanoparticle Characterization and Their Potential as Nanobiofertilizers 552

20.3 Potential of Gold Nanoparticles as Nanobiofertilizer and Their Characterization 554

20.4 Zn Oxide Nanoparticles as Nanobiofertilizer 554

20.5 Actinomycete-Based Nanobiofertilizers 555

20.6 Fungus-Based Nanobiofertilizers 555

20.7 Conclusion 556

References 557

Index 561

عن المؤلف

Charles Oluwaseun Adetunji, Ph D, is a professor in the Department of Microbiology at the Edo University Iyamho, in Edo State, Nigeria. Currently, he is the Director of Intellectual Properties and Technology Transfer and Chairman of the Committee on Research Grants at EUI. He has won several scientific awards and grants from renowned academic bodies such as the Council of Scientific and Industrial Research (CSIR) India. He has published more than 600 papers in peer-reviewed national and international journals as well as more than 50 books, 340 book chapters, and many scientific patents.
Chukwuebuka Egbuna, Ph D, is a chartered chemist and academic researcher. He has been engaged in several roles at New Divine Favor Pharmaceutical Industry Limited, Nigeria as well as at Chukwuemeka Odumegwu Ojukwu University in Nigeria His primary research interests include biochemistry, phytochemistry, pharmacology, etc. He has published research articles in many international journals and edited over 20 books.
Anton Ficai, Ph D, is a professor in the Faculty of Chemical Engineering and Biotechnologies, University Politehnica, Bucharest, Romania. His research interests include tissue engineering, drug delivery systems, multifunctional materials, etc. He has published over 250 scientific papers, edited two books, received 10 patents, and has 18 patents in the application stage.
Oluwatosin Ademola Ijabadeniyi, Ph D, is the founder of Food Safety Africa and is a visiting professor at the Department of Food Science, University of Manitoba, Canada. He has worked in the food industry and academia since 2001 and has conducted research and lectured internationally about food quality and safety. His numerous scientific publications have earned several accolades, including research grants, awards, and fellowships such as the Association of Commonwealth Universities fellowship and the APHL-CDC fellowship.

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