J. M. Graham & D. Rickwood 
Subcellular Fractionation [PDF ebook] 
A Practical Approach

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.