This book constitutes the thoroughly refereedpost-workshop proceedings of the 16th International Workshop on Information Security Applications, WISA 2015, held on Jeju Island, Korea, in August 2015.The 35 revised full papers presented in this volume werecarefully reviewed and selected from 78 submissions. The papers are organizedin topical sections such as hardware security; cryptography, side channelattacks and countermeasures; security and threat analysis; Io T security;network security; cryptography; application security.