Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.
This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
Inhaltsverzeichnis
List of Figures, xix
List of Color Plates, xxiii
List of Tables, xxv
List of Protocols, xxvii
List of Minireviews, xxix
Preface and Acknlowledgments, xxxi
Abbreviations, xxxiii
About the Companion Website xxxviii
1. Introduction, 1
2. Biology of Cultured Cells, 19
3. Laboratory Design and Layout, 37
4. Equipment and Materials, 51
5. Aseptic Technique, 73
6. Safety, Bioethics, and Validation, 89
7. Culture Vessels and Substrates, 111
8. Defined Media and Supplements, 125
9. Serum-Free Media, 149
10. Preparation and Sterilization, 173
10. Supplementary Material* 11. Primary Culture, 207
11. Supplementary Material* 12. Subculture and Cell Lines, 235
13. Authentication and Validation, 259
14. Microbial Contamination, 289
15. Cryopreservation and Banking, 307
16. Cloning and Selection, 327
16. Supplementary Material* 17. Cell Sorting, 347
18. Cell Line Characterization, 359
19. Differentiation, 383
20. Three?]Dimensional Culture 401
21. Scale?]up and Automation, 427
22. Senescence, Immortalization, and Transformation, 455
23. Quantitation, 483
23 Supplementary Material* 24. Cytotoxicity, 513
24. Supplementary Material* 25. Culture of Specific Cell Types, 531
25. Supplementary Material* 26. Stem Cells, 571
26. Supplementary Material* 27. Training Programs, 591
28. Problem Solving 627
29. In Conclusion, 651
Supplementary Material* Appendix III: Sources of Equipment and Material
Appendix IV: Suppliers and Other Resource
Über den Autor
R. IAN FRESHNEY, Ph D, is an honorary Senior Research Fellow at the Instituteof Cancer Sciences at the University of Glasgow in Glasgow, UK. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.
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