This volume focuses on solution and solid-state NMR of carbohydrates, glycoproteins, glyco-technologies, biomass and related topics. It is estimated that at least 80% of all proteins are glycoproteins. Because of the complexity, heterogeneity and flexibility of the sugar chains, the structural biology approaches for glycoconjugates have been generally avoided. NMR techniques although well established for structural analyses of proteins and nucleic acids, cannot be simply applied to this complex class of biomolecules. Nonetheless, recently developed NMR techniques for carbohydrates open the door to conformational studies of a variety of sugar chains of biological interest. NMR studies on glycans will have significant impact on the development of vaccines, adjuvants, therapeutics, biomarkers and on biomass regeneration.
In this volume, the Editors have collected the most up-to-date NMR applications from experts in the field of carbohydrate NMR spectroscopy. Timely and useful, not only for NMR specialists, it will appeal to researchers in the general field of structural biology, biochemistry and biophysics, molecular and cellular biology and material science.
Inhaltsverzeichnis
Intramolecular Hydrogen Bonding in Glycans in Aqueous Solution; NMR Spin-Couplings in Saccharides: Relationships between Structure, Conformation and the Magnitudes of
JHH,
JCH and
JCC Values; Insights into Carbohydrate Recognition by 3D Structure Determination of Protein-Carbohydrate Complexes using NMR; Paramagnetic, RDC and RCSA Constraints in the Structural Analysis of Glycans; Lanthanide-Chelating Carbohydrate Conjugates to Detect Carbohydrate-Protein Interactions; NMR Characterization of the Conformations, Dynamics, and Interactions of Glycosphingolipids; NMR Analysis of Glycosyltransferases; Stable Isotope Labeling of Glycoproteins for NMR Study; Quantifying Carbohydrate Motions Through Solution Measurements: Applications to Immunoglobulin G Fc; Analysis of Glycosaminoglycans by
15N-NMR Spectroscopy; NMR Studies of Protein-Glycosaminoglycan Interactions; Solid-state NMR Analysis of Mannose Recognition by Pradimicin A; Structure and Dynamics of Polysaccharides in Plant Cell Walls From Solid-State NMR; New Methods for the Analysis of Heterogeneous Polysaccharides: Lessons Learned from the Heparin Crisis; NMR Chemical Shift Predictions and Structural Elucidation of Oligo- and Polysaccharides by the Computer Program CASPER; NMR Databases for Plant Cell Wall Biopolymers; Polysaccharides as Major Carbon Sources in Environmental Biodiversity
Über den Autor
Koichi Kato received his Ph.D. in 1991 at Graduate School of Pharmaceutical Sciences, the University of Tokyo under the supervision of Prof. Yoji Arata, and continued his research as Assistant Professor and Lecturer in the same institution. In 2000 he moved to Nagoya City University as full Professor. Since 2006 he has also been a Visiting Professor at the Glycoscience Institute, Ochanomizu University. In 2008 he moved to his current position at Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, holding a Professor position concurrently at Nagoya City University. His research interests include structural analyses of glycoconjugates by NMR spectroscopy and other biophysical and biochemical methods.
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