David Seligson 
Standard Methods of Clinical Chemistry [PDF ebook] 
By the American Association of Clinical Chemists

Standard Methods of Clinical Chemistry, Volume II describes accurate and workable methods used in clinical laboratories. This 21-chapter text covers the principles, reagents, procedure, and calculation of these methods. The opening chapters explore various clinical chemistry methods for determination of serum calcium, cholesterol, chloride, fatty acids, gamma globulin, and hemoglobin. The succeeding chapters review the methods for detection of free and conjugated 17-hydroxycorticosteroids, 17-ketosteroids, serum iron, lipase, nitrogen by Kjeldahl method, and nonprotein nitrogen. The remaining chapters deal with other clinical methods for blood p H, enzyme, phosphatides, urine porphyrins, protein-bound ion, and sulfobromophthalein determination. This book is directed primarily toward clinical chemists, but pathologists, medical technicians, clinical investigators, chemists in other fields, and students will find this book rewarding.