Dinesh Yadav & M. K. Yadav 
Plant Tissue Culture Concepts And Techniques [EPUB ebook] 

Plant Tissue Culture Has Witnessed Several Innovations With The Advances In The Science And Technology Over The Years And Has Substantially Influenced The Growth Of Plant Biotechnology In The Recent Years. This Book Comprises Of 22 Chapters Describing Different Aspects Of Plant Tissue Culture. The First Chapter Introduces The Subject And Gives An Insight Into The Diverse Applications Of The Plant Tissue Culture Followed By Chapter 2 & 3 Highlighting The Experimental Aspects Of Plant Tissue Culture Like Organization Of Lab, Preparation Of Media Etc. The Chapter 4 & 5 Deals With Detail Information About The Plant Growth Regulators Known To Be An Important Component Of Plant Tissue Culture Media, Their Preparation , Biosynthesis And Mode Of Action. The Chapter-6 Describes Different Processes Relevant To Plant Tissue Culture Technique. The Chapter -7 Deals With An Important Issue Of Quality Control In Micropropagation Which Is Rarely Discussed In Books Of Plant Tissue Culture Available. The Chapter-8 Discusses The Relevance, Importance And Limitations Of Plant Tissue Culture For Woody Plants Taking Several Examples While Chapter-9 Deals With The Most Commonly Used Techniques Of Micropropagation. The Chapter-10 Exclusively Describes The Micropropagation Of Carnation Revealing Its Importance While Chapter-11 Highlights The Tissue Culture Technique Relevant For Propagation Of Perennial Helianthus Species. The Chapter-12 Describes The Technique Of Callus Culture From Different Explants, Its Maintenance Taking Brassica As Example. The Chapter-13 Deals With An Important Development Of Plant Tissue Culture Known As Somatic Embryogenesis Highlighting The Technique Of Production Of Somatic Embryos And Development Of Plants. The Chapter-14 Reflects The Practical Application Of Somatic Embryogenesis For Plant Regeneration Taking Rice As An Example. Selection Of Mutants In Plant Tissue Culture Is An Important Issuewhich Has Beenwell Taken In The Chapter-15 Bydescribing Themutant Selection Techniques In Maize Crop. The Protoplast Is An Important Target For Gene Transfer By Electroporation And The Chapter-16 Exclusively Deals With The Technique Of Isolation, Culture And Fusion Techniques. The Production Of Haploidswas Amajor Breakthrough In The Plant Tissue Culture Techniques And Chapter-17 Describes The Technique Of Anther And Microspore Culture. The Chapter-18 Deals With The Preparation Of Artificial Or Synthetic Seeds Using Plant Tissue Culture Techniques While Chapter-19 Highlights Another Important Developments In Plant Tissue Culture I.E. Somaclonal Variation, Its Importance And Methods Of In-Vitro Selection. The Industrial Application Of Plant Tissue Culture Especially For The Production Of Secondary Metabolites Utilizing The Suspension Culture Is Discussed In Chapter-20. There Exist Several Techniques For The Conservation Of Germplasms Along With Plant Tissue Culture Techniques. Based On The Importance Of Conservation Of Germplasms, The Chapter-21 Exclusively Describes The Different Techniques Relevant To Plant Tissue Culture With Potential For Germplasm Conservation. The Advances In The Transgenic Technology Are Based On The Innovation In The Method Of Gene Transfer Predominantly Either By Agrobacterium Mediated Or By Biolistic (Gene Gun) Method. Several Successful Transgenic Plants Have Been Produced Byusing The Agrobacterium Mediated Methods Of Gene Transfer. The Final Chapter- 22 Attempts To Describe The Methods Of Gene Transfer Relevant For Transgenic Technology By Highlighting The Commonly Used Method I.E. Agrobacteriummediated Applicable For Bothmonocots And Dicots.Overall The Book Covers Almost All Aspects Of Plant Tissue Culture Emphasizing On The Practical Aspects Along With Ample Theoretical Information About Eachtopic.