Jiby John Mathew & Ajesh C Kuriakose 
Evaluation of genetic diversity of jackfruit (Artocapus heterophyllus Lam) varieties based on sequence analysis of the rbcL gene [PDF ebook] 

Support

Scientific Study from the year 2016 in the subject Biology – Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Jackfruit (Artocarpus heterophyllus) is commonly grown in home gardens of tropical and sub-tropical countries. The fruit contains high levels of carbohydrates, protein, starch, calcium and vitamins. Jack fruit has diverse medicinal uses, especially anti-oxidant, anti-inflammatory, anti-microbial, anti-cancer and anti-fungal activity. Jackfruit is considered to be an underutilized fruit where most of the fruits get wasted due to ignorance, lack of post harvest technology and gaps in supply chain systems.
Jackfruit contains more protein, calcium, iron, vitamins and other essential nutrients as compared to the common fruits. To find out the morphological variation among jack fruits among Kerala we conducted an elaborative survey and found out the parameters such as tree characteristics, leaf characterises or fruit characteristics of selected samples.
Both qualitative and quantitative data are measured using field visits and standard methods and morphological indicators; after analysis of data there is a morphological variation among the jack fruit samples identified. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers although each technique has advantages and limitations.
The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using the ‘rbc L gene’ based on PCR technique and RFLP markers. The partial sequence of ‘rbc L’ gene of six different Artocarpus varieties was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 651 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 651 sites. The nucleotide frequencies are 27.96% (A), 29.47% (T/U), 19.69% (C), and 22.89% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam has promising leads to further scientific research and livelihood strategies. The study of rbc L gene using PCR and RFLP seems to be a promising tool in establishing genetic diversity among jackfruit varieties.

€29.99
méthodes de payement

A propos de l’auteur

Dr. Prem Jose Vazhacharickal is currently working as an assistant professor in the Department of Biotechnology, Mar Augusthinose College, Ramapuram, Kerala, India. He received the research training and academic guidance from Prof. Dr. Andreas Buerkert, University of Kassel, Germany.

Achetez cet ebook et obtenez-en 1 de plus GRATUITEMENT !
Langue Anglais ● Format PDF ● Pages 75 ● ISBN 9783668383289 ● Taille du fichier 2.7 MB ● Maison d’édition GRIN Publishing ● Lieu München ● Pays DE ● Publié 2017 ● Édition 1 ● Téléchargeable 24 mois ● Devise EUR ● ID 5083433 ● Protection contre la copie sans

Plus d’ebooks du même auteur(s) / Éditeur

1 220 Ebooks dans cette catégorie