An essential guide to biomolecular and bioanalytical techniques and their applications
Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications.
The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories.
* A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods
* Provides the necessary background and skills needed to advance the research methods
* Features a structured approach within each chapter
* Demonstrates an interdisciplinary approach that serves to develop independent thinking
Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.
Table des matières
1. Principles of Health and Safety and Good Laboratory Practice
2. Applications of Chemoinformatics in Drug Discovery
3. Bioinformatics and its Applications in Genomics
4. Gene cloning for the analysis of gene expression
5. Proteomic techniques and their applications
6. Overproduction, Separation and Purification of Affinity-Tagged Proteins from Escherichia coli
7. Chromatography: separation techniques in biology
8. Synthetic Methodology in Chemical Biology
9. Reaction Chemical Kinetics in Biology
10. Mass Spectrometry and its Applications
11. Applications and Complementarity of Analytical Ultracentrifugation and Light-Scattering Techniques
12. Application of isothermal titration calorimetry (ITC) to biomolecular interactions
13. An introduction to Infrared and Raman spectroscopies for pharmaceutical and biomedical studies
14. Fluorescence Spectroscopy and Its Applications in Analyzing Biomolecular Processes
15. Circular Dichroism and related spectroscopic techniques
16. Principles and practice in macromolecular X-ray crystallography
17. Biomolecular NMR Spectroscopy and Structure Determination of DNA
18. Cryo-TEM and biological structure determination
19. Computer modelling and molecular dynamics simulation of biomolecules
A propos de l’auteur
Editor:
Dr Vasudevan Ramesh, retired from the Faculty of the School of Chemistry at the University of Manchester, Manchester, UK, after 18 years of distinguished service. He taught Physical Chemistry at all degree levels and pursued an active research programme in Biomolecular NMR spectroscopy. He was a Leverhulme Research Fellow at the University of Leicester and a Visiting Lecturer and Annual Award winner at the Peking University, Beijing, China.
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