The synergy between synthetic biology and biocatalysis is emerging as an important trend for future sustainable processes. This book reviews all modern and novel techniques successfully implemented in biocatalysis, in an effort to provide better performing enzymatic systems and novel biosynthetic routes to (non-)natural products. This includes the use of molecular techniques in protein design and engineering, construction of artificial metabolic pathways, and application of computational methods for enzyme discovery and design. Stress is placed on current ‘hot’ topics in biocatalysis, where recent advances in research are defining new grounds in enzyme-catalyzed processes. With contributions from leading academics around the world, this book makes a ground-breaking contribution to this progressive field and is essential reading for graduates and researchers investigating (bio)catalysis, enzyme engineering, chemical biology, and synthetic biology.
Table of Content
Genome Mining for Enzyme Discovery; Exploiting Natural Diversity for Industrial Enzymatic Applications; Artificial Metalloenzymes; Computational Enzyme Design: Successes, Challenges, and Future Directions; Computational Techniques for Efficient Biocatalysis; Modulating Enzyme Activity via Incorporation of Non-canonical Amino Acids; Enhancing Enzymatic Performance via Restricted Sequence Space Approaches; Customizing Transcription-factor Biosensors for Modern Biotechnology; Exploiting Biosynthetic Pathways in Fungi: Opportunities for Enhanced or Novel Production; Engineering Enzymes for Natural Product Biosynthesis and Diversification; Impact of Synthetic Biology on Secondary Metabolite Biosynthesis; Self-contained Biocatalysts; Designing Multi-enzymatic Systems for the Preparation of Optically Active Molecules; Artificial Biocatalytic Cascades to Alcohols and Amines; Emerging Fields in One-pot Multi-step Synthesis with Combined Chemo- and Bio-catalysts: Sequential- and Domino-type Process Concepts as well as Compartmentation Strategies; Technical Biocatalysis; Biocatalytic Process Engineering; Enzymes for Detection and Decontamination of Chemical Warfare Agents
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