In this incisive, concise overview of this booming field, the editors — two of the leading figures in the field with a proven track record — combine their expertise to provide an invaluable reference on the topic.
Following a treatment of transcriptome analysis, the book goes on to discuss replacement and mutation analysis, gene silencing and computational analysis. The whole is rounded off with a look at emerging technologies.
Each chapter is accompanied by a concise overview, helping readers to quickly identify topics of interest, while important, carefully selected words and concepts are explained in a handy glossary.
Equally accessible to both experienced scientists and newcomers to the field.
Table of Content
TRANSCRIPTOME ANALYSIS
Single-Cell Expression Profiling
AFLP-based RNA Fingerprinting
The SAGE family: SAGE, Long SAGE and Super SAGE
Deep CAGE: High-Throughput Gene Expression Analysis for Transcription Start Site and Promoter Identification
Paired End di Tagging (GIS-PET) for Transcriptome Characterization
High-Throughput Functional Screening of Genes in planta
Microarrays as Tools to Decipher Transcriptomes in Symbiotic Interactions
Bead Array-based Gene Expression Analysis
Genome-wide Analysis of m RNA expression by Fluorescent Differential Display
Quantitative Real-time RT-PCR
GENE SILENCING, MUTATION ANALYSIS, AND FUNCTIONAL GENOMICS
RNA Interference for Functional Genomics
Extending Functional Genomics: VIGS for Model and Crop Plants
Identification of Expressed Genes by Mutation: TILLING
Identification of Expressed Genes by Mutation: Transposon Tagging
Fast Neutron Mutagenesis
COMPUTATIONAL ANALYSIS
Bioinformatics Tools to Discover Co-expressed Genes in Plants
Promoter Analysis
Integration and Phylogenomics
Structural, Functional, and Comparative Annotation of Plant Genomes
Large-Scale Genomic Sequence Comparisons and Gene Identification with Clust DB
EMERGING TECHNOLOGIES
Nanotechnologies and Fluorescent Proteins for Functional Genomics
Next Generation Sequencing Technologies
454 Sequencing for Functional Genomics
About the author
Gunter Kahl is Professor for Plant Molecular Biology in Frankfurt am Main, Germany. He has served in expert missions for IAEA, FAO and UNESCO across the world and is author of more than 250 scientific publications, including several Wiley-VCH books.
Khalid Meksem is Professor at the Department of Plant, Soil & General Agriculture of Southern Illinois University. He is associate editor of the Journal of Biomedicine and Biotechnology and chaired the Functional Genomic Workshop at the International Plant & Animal Genome Conference in San Diego. He is reviewer for several granting agencies, including the National Science Foundation and the US Department of Agriculture.
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