Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope-skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image.
Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as:
* Illuminators, filters, and isolation of specific wavelengths
* Phase contrast and differential interference contrast
* Properties of polarized light and polarization microscopy
* Fluorescence and confocal laser scanning microscopy
* Digital CCD microscopy and image processing
Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions.
An essential resource for both, experienced and novice microscopists.
विषयसूची
Preface.
Fundamentals of Light Microscopy.
Light and Color.
Illuminators, Filters, and Isolation of Specific Wavelengths.
Lenses and Geometrical Optics.
Diffraction and Interference in Image Formation.
Diffraction and Spatial Resolution.
Phase Contrast Microscopy and Dark-Field Microscopy.
Properties of Polarized Light.
Polarization Microscopy.
Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy.
Fluorescence Microscopy.
Confocal Laser Scanning Microscopy.
Video Microscopy.
Digital CCD Microscopy.
Digital Image Processing.
Image Processing for Scientific Publication.
Appendix I.
Appendix II.
Appendix III.
Glossary.
References.
Index.
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Douglas B. Murphy joined The Johns Hopkins University School of Medicine in 1978, where he presently holds the position of professor of cell biology. Dr. Murphy’s research on cell motility and the assembly and dynamics of cytoskeletal polymers involves extensive use of light and electron microscopy, and for the past 20 years he has taught courses for students and faculty on light microscopy, confocal fluorescence microscopy and EM. He is co-founder and director of an institution-wide School of Medicine Microscope Facility that provides microscope services to over 100 research laboratories.
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