This updated edition explores techniques in identifying and characterizing various skin stem cells and generating skin-like structures in vitro. The goal of the book is to present exciting advances in skin biology in an effort to provide a better understanding of skin and, eventually, to allow us to regenerate skin with its original form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Skin Stem Cells: Methods and Protocols, Third Edition serves as a valuable assemblage of protocols for researchers already working in the field and for those who now wish to newly embark on studies of skin stem cells.