This volume details a collection of state-of-art methods including identification of novel nc RNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific nc RNAs, computational methods and workflows for nc RNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for nc RNA analyses, network-based methods, and kinetic modelling of nc RNA-mediated gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Computational Biology of Non-Coding RNA: Methods and Protocols aims to provide a state-of-the-art collection of computational methodsand approaches that will be of value to researchers interested in nc RNA field.
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