A complete examination of the uses of the atomic force microscope
in biology and medicine
This cutting-edge text, written by a team of leading experts, is
the first detailed examination of the latest, most powerful
scanning probe microscope, the atomic force microscope (AFM). Using
the AFM, in combination with conventional tools and techniques,
readers gain a profound understanding of the cell, subcellular
organelles, and biomolecular structure and function.
The text begins with three chapters describing the molecular
machinery and mechanism of cell secretion and membrane fusion in
cells, using approaches that combine AFM, electron microscopy,
X-ray diffraction, photon correlation spectroscopy, molecular
biology, biochemistry, and electrophysiology. The discovery of a
new cellular structure the ‘porosome’ or fusion pore–the cells
secretory machinery, the molecular mechanism of membrane fusion in
cells, and the expulsion of intravesicular contents during cell
secretion are outlined in the first three chapters. The book also
covers:
* Identification of the ‘porosome’ in the growth hormone secreting
cell of the pituitary gland
* Probing the structural and physical properties of microbial cell
surfaces
* Scanning probe microscopic characterization of the higher plant
cell wall and its components
* Case studies of nano drug delivery systems using engineered
dendrimers
* AFM techniques for studying living cells
* Investigating the intermolecular forces of leukocyte adhesion
molecules
* Protein-protein interactions
* Micromechanical properties of lipid bilayers and vesicles
The text concludes with four chapters that examine new and emerging
approaches in the use of force microscopy in biology and
medicine.
This text is ideal for advanced undergraduate and graduate students
and researchers in cell and molecular biology, genetics, genomics,
physiology, neuroscience, biophysics, and biochemistry. Not only
does it provide the theory, but also practical considerations such
as the selection of the right tools and approach.
Daftar Isi
Preface.
Contributors.
Chapter 1. Porosome: The Universal Secretory Machinery in Cells
(Bhanu P. Jena).
Chapter 2. Molecular Mechanism of SNARE-Induced Membrane Fusion
(Bhanu P. Jena).
Chapter 3. Molecular Mechanism of Secretory Vesicle Content
Expulsion During Cell Secretion (Bhanu P. Jena).
Chapter 4. Fusion Pores in Growth-Hormone-Secreting Cells of the
Pituitary Gland: An AFM Study (Lloyd L. Anderson and Bhanu P.
Jena).
Chapter 5. Properties of Microbial Cell Surfaces Examined by
Atomic Force Microscopy (Yves F. Dufre&ncirc;e).
Chapter 6. Scanning Probe Microscopy of Plant Cell Wall and Its
Constituents (Ksenija Radotić, Miodrag
Mićić, and Milorad
Jeremić).
Chapter 7. Cellular Interactions of Nano Drug Delivery Systems
(Rangaramanujam M. Kannan, Omathanu Pillai Perumal, and
Sujatha Kannan).
Chapter 8. Adapting AFM Techniques for Studies on Living Cells
(J. K. Heinrich Hörber)
Chapter 9. Intermolecular Forces of Leukocyte Adhesion Molecules
(Xiaohui Zhang and Vincent T. Moy).
Chapter 10. Mechanisms of Avidity Modulation in Leukocyte
Adhesion Studied by AFM (Ewa P. Wojcikiewicz and Vincent T.
Moy).
Chapter 11. Resolving the Thickness and Micromechanical
Properties of Lipid Bilayers and Vesicles Using AFM (Guangzhao
Mao and Xuemei Liang).
Chapter 12. Imaging Soft Surfaces by SFM (Andreas Janke and
Tilo Pompe).
Chapter 13. High-Speed Atomic Force Microscopy of Biomolecules
in Motion (Tilman E. Schäffer).
Chapter 14. Atomic Force Microscopy in Cytogenetics (S.
Thalhammer and W. M. Heckl).
Chapter 15. Atomic Force Microscopy in the Study of
Macromolecular Interactions in Hemostasis and Thrombosis: Utility
for Investigation of the Antiphospholipid Syndrome (William J.
Montigny, Anthony S. Quinn, Xiao-Xuan Wu, Edwin G. Bovill, Jacob H.
Rand, and Douglas J. Taatjes).
Index
Tentang Penulis
BHANU P. JENA, PHD, DSc, is the George E. Palade University
Professor and Distinguished Professor of Physiology at Wayne State
University School of Medicine, Detroit, Michigan. In addition, Dr.
Jena is Director of the University’s Nano Bio Science Institute.
J. K. HEINRICH HÖRBER, Ph D, is Professor in the
Department of Physics at University of Bristol, Bristol, UK.
Ebook lainnya dari penulis yang sama / Editor
2,696 Ebooks dalam kategori ini
