Eric May & Mark Jones 
Heritage Microbiology and Science [PDF ebook] 
Microbes, Monuments and Maritime Materials

Introduction: Heritage Microbiology, Science And the Mary Rose: What are we trying to achieve? Introduction; Background; Conference Themes and the Mary Rose; What are we trying to achieve? Conclusion; Part 1: Heritage Monuments and Materials; Heritage Research and Practice: Towards a better understanding? Introduction; Evaluation of Biodeterioration Processes; Biodeterioration Mechanisms; Exogenic Parameters; Biofilm – A Stabilising Microniche; Environmental Conditions for Biodeterioration Processes; Microbiological Assessment of Biodeterioration Impacts; Microbiology and Archaeology – Case Studies; Terracotta Army in Xian / China (Polychrome Coatings); Nydam Mose / Denmark (Metals); Temple of Angkor Wat, Cambodia (Natural Stone); Archaeological Site of Milet in Turkey (Waterlogged Marble); Prospective Needs for an Interdisciplinary Approach in Conservation Microbiology; Mapping Decay: GIS, Microbes and Stone Degradation across Scales; Introduction; Geographical Information Systems; Use of Images for Classifying Degradation; Illustrations of Mapping Degradation in a GIS; Conclusions; Microbial Colonisation of Historic Buildings in Latin America; Introduction; Methods; Sites and Sampling; Detection and Identification; Results and Discussion Analysis of Bacterial Communities on an Antique Stained Glass Window; Introduction; Methods and Results; Deterioration on the window ‘NativitÓ’; Sampling, Growth Conditions and Phenotypical Characterization; Molecular Characterization; Conclusions; Assessing the Suitability of Novel Biocides for use on Historic Surfaces; Introduction; Methods and Results; Compatibility of Novel Treatments with Conservation Products; Tests on Sandstone; Accelerated Weathering; Tests on Traditional Painted Plaster; Field Studies; Test Surfaces; Test Rigs; Treatments; Effectiveness Measurements; Fluorescence and Colour Measurements after Treatment Application; Post-Treatment Assessment; The Occurrence of Heterotrophic Microorganisms on Heritage Surfaces; Discussion; Conclusion; Biocalcification: The Context for Bioremediation; Introduction; Biomineralization; Calcium Carbonate Biomineralization; Monumental Stone Decay and Conservation; Bioremediation for Conservation; Methods and Results; On-site Application; Bio-inducing Macromolecules Solutions (BIMSs); Methodology of Application; BMT Evaluation; On-site results; Conclusions; The Biobrush Project for Bioremediation of Heritage Stone; A Need for Stone Conservation; Rationale for Biobrush Research; Collection and Analysis of Encrusted Stone from Historic Buildings; Selection and Screening of Bacterial Cultures for use in Bioremediation; Evaluation of Delivery Systems to carry Biological Agents onto the Stone; Mineral Changes in Stones during Bioremediation Treatment; Field Trials of Bioremediation on Buildings and Monuments; Recommended Methodologies; Implications of Biobrush Research; Part 2: Molecular Methods for Heritage Artefacts and Monuments; Molecular Studies for Cultural Heritage: State of the Art; Introduction; Previous Methods for the Detection of Microorganisms; Culture-Independent Methods to Detect Microorganisms; Recent Advancements for Detecting Microorganisms; MDA-PCR Amplifications; DNA Library Screening; Detection and Analysis of Chimeras; Analysis Based on DNA and RNA; Further Perspectives; Bacteria in Archaeological and Waterlogged Wood: Molecular Protocols for Diversity and Community Studies; Introduction; Waterlogged Wood and its Microbiology; Extraction of Nucleic Acids from Wood; Overcoming Low Quantities of Impure Nucleic Acids; PCR and the Separation of Mixed-Origin PCR Products; Fluorescent In Situ Hybridisation and Waterlogged Wood; Conclusions; Synchrotron Radiation for the Investigation of Objects of Cultural Heritage Value; Introduction; Synchrotron Radiation; Examples of Synchrotron Science as Applied to Heritage Materials; Archaeological Iron; Erosion of Carbonate Building Materials; Textile Fibres from the Qumran Caves; Corinthian Style Helmet from Ancient Greece; Conclusions; Summary; Fluorescent In Situ Hybridization (FISH) as Molecular Tool to Study Bacteria causing Biodeterioration; Introduction; FISH Applied to the Study of Biodeterioration of Works of Art; Limits and Advantages of FISH to Study Microbial Communities associated with Biodeterioration; Methods that Enhance the Signal; Identification of Bacteria from Waterlogged Archaeological Wood; Introduction; Method and Results; Sampling; Cultivation; DNA-Based Identification; DNA Extraction; PCR Amplification and Construction of 16S r DNA Clone Libraries and T-RFLP Community Fingerprinting; Sequencing of 16S r DNA Inserts and Phylogenetic Analysis; Conclusions; Summary; Novel Combined Approach Based on Phospholipid Fatty Acids and 16S-r DNA PCR-SSCP Analyses to Characterise Fouling Biofilms on Historic Monuments; Introduction; Culture-Independent Approaches to Characterise Microbial Communities; Materials and Methods; Site Description; Sample Collection; Biomarker Analysis; DNA Extraction, PCR-SSCP and Sequencing of DNA; Digital Image Analysis; Nucleic Acid and Phylogenetic Analyses Results; Biofilm Biomass and PLFA Profiles; Discussion; Biomass and Diversity of Epilithic Biofilms; Conclusions; On the Use of 23S r RNA Gene Sequences to Assess a High Diversity of Acidobacteria in Altamira Cave; Introduction; Materials and Methods; Sampling and DNA Extraction; Amplification of Acidobacterial r DNA and Construction of Clone Libraries; Phylogenetic Analyses and Tree Reconstruction based on r DNA Sequences; Results and Discussion; Conclusions; Part 3: Historic Ships and their Preservation; The In-Situ Preservation of Archaeological Sites Underwater: An Evaluation of some Techniques; Introduction; Why In-Situ Preservation? Threats to Underwater Archaeological Heritage; Measuring the Extent of Deterioration; Examples of Techniques used for In-Situ Protection; The ‘Polders’, The Netherlands; Red Bay, Canada; The Bzn Wrecks, The Netherlands; The Darsser Cog, Germany; The Avondster, Sri Lanka; Roman Quay, The Netherlands; Thirteenth Century Wreck, Denmark; William Salthouse, Australia; The Zakynthos Wreck, Greece; Colossus, United Kingdom; James Matthews, Australia; Fredericus (Raar-Project), Sweden; Conclusions and Future Directions; Molecular Bacterial Diversity in the Timbers of the Tudor Warship the Mary Rose; Introduction; Role of Bacteria in the Sulfur and Iron Cycles; Molecular Diversity of Bacteria associated with Buried and Raised Ship Timbers; Conclusions; Timber Conservation on Nelson’s Flagship HMS Victory; Introduction; Original Construction; The Early Years; Into Drydock 1922; Restoration and Repair in the 1920s; The 1955-64 Great Repair; Completion of the Great Repair 1964 – 2000; Timber Supply; Concluding Comments; Summary; Informing the Conservation, Display and Long-Term Preservation of the HMS Victory Trafalgar Sail; Introduction; The Performance of the Canvas; Conservation and Display; Condition Monitoring; Nuclear Magnetic Relaxometry; Near Infrared Spectroscopy (NIR); Conclusions; Extraction of Iron Compounds from Waterlogged Pine Wood from the Vasa; Introduction; Materials and Methods; Chemicals; Extraction Procedures; Analyses; Results and Discussion; Co-Extraction of other Compounds; Effects on the Wood; Conclusions; Summary; Electrolysis in the Conservation of Large Artefacts: The M33 and the s.v.Cutty Sark; Introduction; Soak Treatment; Electrolytic Treatment; Electrolysis in Conservation; Electrolysis of the M33; Electrolysis of the s.v. Cutty Sark; Conclusions; Summary; Desiccated Storage of Chloride-Contaminated Iron: A Study of the Effects of Loss of Environmental Control; Introduction; Corrosion and Electrolytes; ss Great Britain: A big Corrosion Problem; Implementation of Environmental Control; Chloride-Infested Iron: Corrosion and Corrosion Products; Chloride on the Iron Hull of the ss Great Britain; Modelling Iron Corrosion during Drying of Chloride-Infested Iron; Environmentally-Controlled Storage in Practice; Experimental; Results; Fecl2. 4h2o/ Iron Powder Mix: 15%-22% Relative Humidity (Figure 4); Fecl2.4h2o/ Iron Powder Mix: 15%-30% Relative Humidity (Figure 5); -Feooh/Iron Powder Mix: 15%-22% Relative Humidity (Figure 6); Feooh/Iron Powder Mix: 15%-30% Relative Humidity (Figure 7); Fecl2. 4h2o/Iron Powder Mix: 22%- 65% Relative Humidity (Figure 8); Discussion; Endnote; Microbiology and Art: An Education Opportunity; Introduction; Applied Microbiology; Microbiology and Art; Deterioration of Art; Beauty of Microorganisms; Microorganisms in Art; Combining Microbiology and Art; Microbiology And…; Concluding Remarks

Eric May is Reader in Microbiology at the School of Biological Sciences, University of Portsmouth. He has been known for his work on the role of bacteria in stone deterioration for 20 years but also recently coordinated an EU study to assess the value of biotechnology for remediation of altered stone in buildings (BIOBRUSH). He chaired the scientific committees and organised the international heritage meeting Heritage Microbiology and Science (HMS 2005) in Portsmouth in June 2005 along with Mark Jones at the Mary Rose Trust. He is co-editor of Conservation Science: Heritage Materials and was a participant in a recent Preserving the Past research initiative that looked at the methods used in heritage and conservation work. Mark Jones is Head of Collections at the Mary Rose Trust. A leading conservation scientist, involved with the Mary Rose Trust since 1983, Dr Jones devised the conservation methodology for the hull and wooden artefacts at the Mary Rose Trust and is now responsible for all conservation matters. His principle research interests lie in the structure and degradation of archaeological wood, acid problem in treated and untreated archaeological wood and his conservation interests include the stabilisation of large waterlogged wooden objects. He is also responsible for the conservation of the Dover Bronze-Age Boat, a third century Gallo-Celtic Boat from Guernsey, the tenth century Graveney Boat for the National Maritime Museum and numerous pre-historic logboats. Dr Jones also leads a team of textile conservators working to conserve the fore-top sail of HMS Victory for display to the general public. Julian Mitchell is a Senior Lecturer in Microbial Genetics at the School of Biological Sciences, University of Portsmouth. He is a molecular biologist with an interest in the application of molecular techniques to the study of microbes in the environment.