This greatly expanded and updated edition of a classic reference
work comprises two volumes offering a compendium of methods for
multiplying orchids through micropropagation.
* A detailed collection of procedures and methods for multiplying
orchids, including organ, tissue, and cell culture techniques in
vitro
* Presents classic techniques that have been in the forefront of
orchid propagation since they were first developed in 1949
* Detailed procedures are appended with tables and complete
recipes for a large number of culture media
* Includes many illustrations, chemical formulas, historical
vignettes, and seldom seen illustrations of people, orchids,
apparatus and tools
‘… an excellent resource like its predecessor,
…both informative and captivating, and served as a reminder
of why we go to such extremes in our quest to propagate these
plants.’ American Orchid Society, 2009
‘…in the sense of its universal value and
importance, this Second Edition will undoubtedly be considered a
classic, if only because it will serve as a sole and invaluable
resource on the subject.’ Plant Science Bulletin,
2009
Daftar Isi
Preface.
Preface to the First Edition.
1. History.
Terminology.
Origins of Orchid Micropropagation.
Culture of Tissues and Organs.
First Micropropagation of Orchids.
The Second Aseptic Culture of an Orchid Explant.
Plant Diseases and Meristems.
The Third Aseptic Culture of an Orchid Explant.
The Fourth Aseptic Culture of an Orchid Explant.
Who Pioneered Micropropagation?.
Root Cultures.
Leaf Cultures.
Stems.
Flower Buds, Flowers, Floral Segments, and Reproductive Organs.
Inflorescences.
A Patent.
Doubtful Claims.
Mutations.
Theft in Vitro.
Darkening of Culture Media.
Anticontaminants.
Cell and Protoplast Culture.
Flowering in Vitro.
The Future.
2. General Outline of Techniques and Procedures.
Media Components.
Stock Solutions.
State of the Medium.
Sterlization.
Surface Decontamination.
Preparation of the Medium.
Culture Vessels.
Culture Conditions.
Placing Plant Material in Culture.
Internal Contaminants.
Enzymes for Protoplast Isolation.
Work Area.
Washing Glassware.
3. Methods for Specific Genera.
Acampe.
Aeridachnis.
Aerides.
Anacamptis.
Angraecum.
Anoectochilus.
Arachnis.
Arachnostylis.
Aranda.
Aranthera.
Arundina.
Ascocenda.
Ascocentrum.
Ascofinetia.
Barlia.
Bletilla.
Brassavola.
Brassia.
Brassocattleya.
Brassolaeliocattleya.
Bulbophyllum.
Burkillara.
Caladenia.
Calanthe.
Catasetum.
Cattleya.
Cleisostoma.
Clowesia.
Cymbidium.
Cypripedium.
Cyrtopodium.
Dactylorchis.
Dactylorhiza.
Darwinara.
Dendrobium.
Disa.
Diuris.
Doriella.
Doritaenopsis.
Doritis.
Encyclia.
Epidendrum.
Epiphronitis.
Eulophia.
Gastrochilus.
Geodorum.
Goodyera.
Grammatophyllum.
Habenaria.
Hetaeria.
Holttumara.
Ipsea.
Kagawara.
Laelia.
Laeliocattleya.
Liparis.
Ludisia (Haemaria).
Luisia.
Lycaste.
Malaxis.
Maxillaria.
Miltonia.
Mokara.
Mormodes.
Neofinetia.
Neostylis.
Neottia.
Nervilia.
Nigritella.
Oberonia.
Odontioda.
Odontoglossum.
Odontonia.
Oncidium.
Ophrys.
Orchis.
Otochilus.
Pachystoma.
Paphiopedilum.
Papilionanthe.
Phaius.
Phalaenopsis.
Pholidota.
Phragmipedium.
Pleione.
Pogonia.
Potinara.
Renades.
Renanetia.
Renantanda.
Renanthera.
Restrepia.
Rhynchostele.
Rhynchostylis.
Saccolabium.
Sarcanthus.
Satyrium.
Schomburgkia.
Serapias.
Sophrolaeliocattleya.
Spathoglottis.
Spiranthes.
Thelymitra.
Thunia.
Trudelia.
Vanda.
Vandofinetia.
Vanilla.
Vascostylis.
Vuylstekeara.
Zygopetalum.
4. Summary.
Culture Conditions.
Media Components.
Applications of Orchid Tissue Culture.
Negative Aspects of Micropropagation.
References.
Appendix 1: General Information on Supplies, Equipment, Terms, and Reagents.
Appendix 2: Sources of Supplies and Equipment.
Appendix 3: Some Sites of Interest on the World Wide Web.
Appendix 4: Light.
Appendix 5: Formulary.
Appendix 6: Atomic Weights, Concentrations, Exponents, Greek and Roman Letters, Ions, Measurements, Molecular Weights, Prefixes, Valences, Units, and Solutions.
Appendix 7: Additional Information.
Appendix 8: Plant Preservative Mixture.
Glossary.
Index.
Title Page of the First Edition
Tentang Penulis
Dr Joseph Arditti majored in floriculture as an undergraduate and received his doctorate from the University of Southern California in 1965. After serving as a lecturer for one year at the University of Southern California, he accepted a faculty position at the University of California, Irvine in 1966 where he taught General Botany, Horticulture, and Plant Physiology while engaging in research on various aspects of orchid biology in the US, Indonesia, Singapore, and Malaysia. Dr Arditti retired in 2001 and is now Professor Emeritus. He continues to write and travel worldwide to lecture at scientific meetings and to orchid groups. He is acknowledged to be one of the world’s leading experts on orchid biology and propagation.