This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.
Daftar Isi
Preface vii
Contributors ix
List of Abbreviations xiii
1. Growth of Human Lung Tumor Cells in Culture 1
Reen Wu
2. Culture of Normal and Malignant Gastric Epithelium 23
Jae-Gahb Park, Ja-Lok Ku, Hee-Sung Kim, So-Yeon Park and Michael J.Rutten
3. Establishment of Cell Lines from Colon Carcinoma 67
Robert H. Whitehead
4. Pancreatic Cancer-Derived Cultured Cells: Genetic Alterations and Application t o an Experimental Model of Pancreatic Cancer Metastasis 81
Haruo Iguchi, Kazuhiro Mizumoto, Masaki Shono, Akira Kono and Soichi Takiguchi
5. The Establishment and Characterization of Bladder Cancer Cultures In Vitro 97
Vivian X. Fu, Steven R. Schwarze, Catherine A. Reznikoff and David F. Jarrard
6. Long-term Culture of Normal and Malignant Human Prostate Epithelial Cells 125
Robert K. Bright and Jennifer D. Lewis
7. The Development of Human Ovarian Epithelial Tumor Cell Lines from Solid Tumors and Ascites 145
Anne P.Wilson
8. Culture of Cervical Carcinoma Tumor Cell Lines 179
Peter Stern, Catherine West and Deborah Burt
9. Primary Culture of Human Mammary Tumor Cells 205
Valerie Speirs
10. Myoepithelium: Methods of Culture and Study 221
Sanford H. Barsky and Mary L. Alpaugh
11. Multistage Head and Neck Squamous Cell Carcinoma 261
Kirsten G. Edington, lsabella J. Berry, Margaret O’Prey, Julie E. Burns, Louise J. Clark, Roy Mitchell, Gerry
Robertson, David Soutar, Lesley W. Coggins and E. Kenneth Parkinson
12. Culture of Melanocytes from Normal, Benign, and Malignant Lesions 289
Ruth Halaban
13. Establishment and Culture of Human Leukemia-Lymphoma Cell Lines 319
Hans G. Drexler
14. In Vitro Culture of Malignant Brain Tumors 349
John L. Darling
15. Culture of Human Neuroendocrine Tumor Cells 373
Roswitha Pfragner, Annemarie Behmel, Elisabeth lngolic and Gerhard H. Wirnsberger
Suppliers 405
Index 419
Tentang Penulis
R. Ian Freshney gained a B.Sc. (1960) and Ph.D. (1964) from Glasgow University, where he is on the faculty of Medical Oncology as a senior lecturer and an Honorary Senior Research Fellow. Dr. Freshney currently teaches a number of international basic and specialized cell culture courses, and his research interests include human tumor culture. He is the author of Culture of Animal Cells, a Manual of Basic Technique, and has edited numerous other books on specialized cell culture.
Roswitha Pfragner, Ph.D., studied biology at the University of Vienna, Austria and is currently a senior lecturer at the Institute of Pathophysiology. Her research interests include human neuroendocrine tumors, multiple endocrine neoplasia, drug resistance, apoptosis, and genetic events in tumorigenesis.
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