With extensive coverage of synthesis techniques and applications,
this text describes chemical biology techniques which have gained
significant impetus during the last five years. It focuses on the
methods for obtaining modified and native nucleic acids, and their
biological applications. Topics covered include:
* chemical synthesis
of modified RNA
* expansion of the genetic alphabet in nucleic acids by creating
new base pairs
* chemical biology of DNA replication: probing DNA polymerase
selectivity mechanisms with modified nucleotides
* nucleic-acid-templated chemistry
* chemical biology of peptide nucleic acids (PNA)
* the interactions of small molecules with DNA and RNA
* the architectural modules of folded RNAs
* genesis and biological applications of locked nucleic acid
(LNA)
* small non-coding RNA in bacteria
* micro RNA-guided gene silencing
* nucleic acids based therapies
* innate immune recognition of nucleic acid
* light-responsive nucleic acids for the spatiotemporal control
of biological processes
* DNA methylation
* frameworks for programming RNA devices
* RNA as a catalyst: The Diels-Alderase-Ribozyme
* evolving an understanding of RNA function by in vitro
approaches
* the chemical biology of aptamers: synthesis and
applications
* nucleic acids as detection tools
* bacterial riboswitch discovery and analysis
The Chemical Biology of Nucleic Acids is an
essential compendium of the synthesis of nucleic acids and
their biological applications for bioorganic chemists, chemical
biologists, medicinal chemists, cell biologists, and molecular
biologists.
Tabella dei contenuti
Foreword vii
Preface ix
List of Contributors xi
1 Chemical Synthesis of Modified RNA 1
Claudia Höbartner and Falk Wachowius
2 Expansion of the Genetic Alphabet in Nucleic Acids by Creating New Base Pairs 39
Ichiro Hirao and Michiko Kimoto
3 Chemical Biology of DNA Replication: Probing DNA Polymerase Selectivity Mechanisms with Modified Nucleotides 63
Andreas Marx
4 Nucleic Acid-templated Chemistry 73
Michael Oberhuber
5 Chemical Biology of Peptide Nucleic Acids (PNAs) 103
Peter E. Nielsen
6 The Interactions of Small Molecules with DNA and RNA 115
Yun Xie, Victor K. Tam and Yitzhak Tor
7 The Architectural Motifs of Folded RNAs 141
Valérie Fritsch and Eric Westhof
8 Genesis and Biological Applications of Locked Nucleic Acids (LNAs) 175
Harleen Kaur and Souvik Maiti
9 Small Non-coding RNA in Bacteria 199
Sabine Brantl
10 Micro RNA-guided Gene Silencing 223
Gunter Meister
11 Nucleic Acid-based Therapies 233
Britta Hoehn and John J. Rossi
12 Innate Immune Recognition of Nucleic Acids 261
Stefan Bauer
13 Light-responsive Nucleic Acids for the Spatiotemporal Control of Biological Processes 279
Alexander Heckel and Günter Mayer
14 DNA Methylation 307
Albert Jeltsch and Renata Z. Jurkowska
15 Frameworks for Programming RNA Devices 323
Maung Nyan Win, Joe C. Liang and Christina D. Smolke
16 RNA as a Catalyst: The Diels-Alderase Ribozyme 339
Andres Jäschke
17 Evolving an Understanding of RNA Function by In Vitro Approaches 355
Qing Wang and Peter J. Unrau
18 The Chemical Biology of Aptamers: Synthesis and Applications 377
Günter Mayer and Bernhard Wulffen
19 Nucleic Acids as Detection Tools 401
Jeffrey C.F. Lam, Sergio Aguirre and Yingfu li
20 Bacterial Riboswitch Discovery and Analysis 433
Tyler D. Ames and Ronald R. Breaker
Index 455
Circa l’autore
Günter Mayer, Assistant Professor, LIMES Prog. Unit Chemical Biology and Medicinal Chemistry, Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Germany
Professor Mayer’s research involves molecular diversity applied to elucidate the conformation, function and identity of biomolecules. He is a co-founder of Nasca Cell Technologies AG, a leading service provider of innovative biomolecules, where he headed the combinatorial biotechnology department. He has published papers in leading journals such as Nature, Angewandte Chemie and Journal of the American Chemical Society.
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