Huimin Zhao 
Protein Engineering [EPUB ebook] 
Tools and Applications

Part I – Directed Evolution
1 – Continuous Evolution of Proteins In Vivo
2 – In Vivo Biosensors for Directed Protein Evolution
3 – High-Throughput Mass Spectrometry Complements Protein Engineering
4 – Recent Advances in Cell Surface Display Technologies for Directed Protein Evolution
5 – Iterative Saturation Mutagenesis for Semi-rational Enzyme Design
Part II – Rational and Semi-Rational Design
6 – Data-driven Protein Engineering
7 – Protein Engineering by Efficient Sequence Space Exploration Through Combination of Directed Evolution and Computational Design Methodologies
8 – Engineering Artificial Metalloenzymes
9 – Engineered Cytochrome P450s for Biocatalysis
Part III – Applications in Industrial Biotechnology
10 – Protein Engineering Using Unnatural Amino Acids
11 – Application of Engineered Biocatalysts for the Synthesis of Active Pharmaceutical Ingredients (APIs)
12 – Directing Evolution of the Fungal Ligninolytic Secretome
13 – Engineering Antibody-Based Therapeutics: Progress and Opportunities
14 – Programming Novel Cancer Therapeutics: Design Principlesfor Chimeric Antigen Receptors
Part IV – Applications in Medical Biotechnology
15 – Development of Novel Cellular Imaging Tools Using Protein Engineering

Dr. Huimin Zhao is the Steven L. Miller Chair of chemical and biomolecular engineering, and professor of chemistry, biochemistry, biophysics, and bioengineering at the University of Illinois at Urbana-Champaign (UIUC). He received his B.S. degree in Biology from the University of Science and Technology of China in 1992 and his Ph.D. degree in Chemistry from the California Institute of Technology in 1998 under the guidance of Dr. Frances Arnold. Prior to joining UIUC in 2000, he was a project leader at the Industrial Biotechnology Laboratory of the Dow Chemical Company. He was promoted to full professor in 2008. Dr. Zhao served as a consultant for over 10 companies such as Pfizer, Maxygen, BP, Gevo, and zu Chem, and a Scientific Advisory Board member of Gevo, Myriant Technologies, Toulouse White Biotechnology (TWB) and Agri Metis. He was a member of National Academies’ study group on Industrialization of Biology: A Roadmap to Accelerate Advanced Manufacturing of Chemicals. Dr. Zhao has authored and co-authored over 260 research articles and over 20 issued and pending patent applications with several being licensed by industry. In addition, he has given plenary, keynote or invited lectures in over 290 international meetings, universities, industries, and research institutes. His primary research interests are in the development and applications of synthetic biology tools to address society’s most daunting challenges in health, energy, and sustainability, and in the fundamental aspects of enzyme catalysis, cell metabolism, and gene regulation.