Mahmoud H. Hamdan & Pier G. Righetti 
Proteomics Today [PDF ebook] 
Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis, and Microarray Technology

The last few years have seen an unprecedented drive toward the
application of proteomics to resolving challenging biomedical and
biochemical tasks. Separation techniques combined with modern mass
spectrometry are playing a central role in this drive. This book
discusses the increasingly important role of mass spectrometry in
proteomic research, and emphasizes recent advances in the existing
technology and describes the advantages and pitfalls as well.

* Provides a scientifically valid method for analyzing the
approximatey 500, 000 proteins that are encoded in the human
genome

* Explains the hows and whys of using mass spectrometry in
proteomic analysis

* Brings together the latest approaches combining separation
techniques and mass spectrometry and their application in proteome
analysis

* Comments on future challenges and how they may be addressed

* Includes sections on troubleshooting

Tabella dei contenuti

PREFACE TO PART I.

ACKNOWLEDGMENT.

I: INTRODUCTION TO PART I.

1. INSTRUMENTATION AND DEVELOPMENTS.

1.1 Introduction.

1.2 Ionization Techniques for Macromolecules.

1.3 Examples on Analytical Solutions Based on FAB-MS.

1.4 Electrospray Ionization.

1.5 Matrix-Assisted Laser Desorption Ionization.

1.6 Ion Detection.

1.7 Types of Analyzers.

1.8 Hybrid Analyzers.

1.9 Tandem Mass Spectrometry.

1.10 Current MS Instrumentation in Proteome Analyses.

1.11 Current MS-Based Proteomics.

1.12 Recent Achievements and Future Challenges.

1.13 Concluding Remarks.

References.

2. PROTEOMICS IN CANCER RESEARCH.

2.1 Introduction.

2.2 Pancreatic Ductal Adenocarcinoma.

2.3 Proteomic Analysis of Human Breast Carcinoma.

2.4 Proteomic Profiling of Chemoresistant Cancer Cells.

2.5 Signal Pathway Profiling of Prostate Cancer.

2.6 Emerging Role of Functional and Activity-Based Proteomics in
Disease Understanding.

2.7 Activity-Based Protein Profiling.

2.8 Probing Protein Functions Using Chromophore-Assisted Laser
Inactivation.

2.9 Role of Protein-Tyrosine Kinases.

2.10 Concluding Remarks and Future Prospects.

References.

3. CURRENT STRATEGIES FOR PROTEIN QUANTIFICATION.

3.1 Introduction.

3.2 Global Internal Standard Technology.

3.3 Differential In-Gel Electrophoresis.

3.4 Quantification of Modified Proteins.

3.5 Comments and Considerations.

3.6 Other Approaches.

3.7 Emerging Role of Microfluidic Devices.

3.8 Concluding Remarks.

References.

II: PROTEOMICS TODAY: SEPARATION SCIENCE AT WORK.

4. CONVENTIONAL ISOELECTRIC FOCUSING IN GEL MATRICES AND
CAPILLARIES AND IMMOBILIZED p H GRADIENTS.

4.1 Introduction.

4.2 Conventional Isoelectric Focusing in Amphoteric Buffers.

4.3 Immobilized p H Gradients.

4.4 Capillary Isoelectric Focusing.

4.5 Separation of Peptides and Proteins by CZE in Isoelectric
Buffers.

4.6 Conclusions.

References.

5. SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL
ELECTROPHORESIS.

5.1 Introduction.

5.2 SDS-Protein Complexes: a Refinement of the Model.

5.3 Theoretical Background of Mr Measurement by
SDS-PAGE.

5.4 Methodology.

5.5 Gel Casting and Buffer Systems.

5.6 Blotting Procedures.

5.7 Conclusions.

References.

6. TWO-DIMENSIONAL MAPS.

6.1 Introduction.

6.2 Some Basic Methodology Pertaining to 2D PAGE.

6.3 Prefractionation Tools in Proteome Analysis.

6.4 Multidimensional Chromatography Coupled to MS.

6.5 Protein Chips and Microarrays.

6.6 Nondenaturing Protein Maps.

6.7 Spot Matching in 2D Gels via Commercial Software.

6.8 Conclusions.

References.

INDEX.

Circa l’autore

MAHMOUD HAMDAN, Ph D, is the head of Mass Spectrometry &
Separation Technologies, Glaxo Smith Kline, Verona, Italy.

PIER GIORGIO RIGHETTI, Ph D, is Professor of Biochemistry and
director of the Laboratory of Proteome Science at the University of
Verona, Italy.