Marcelo Ricardo Romero & Felipe Montero 
Synthesis of chromatography adsorbent immobilized metal affinity (IMAC) from agarose and effect of the amount of NaBH4 in the derivatization step [PDF ebook] 

Scientific Essay from the year 2014 in the subject Chemistry – Materials Chemistry, grade: 10, University of Córdoba, language: English, abstract: A synthesis of IMAC adsorbent was performed. Agarose matrix was derivatized with epichlorohydrin using different amounts of Na BH4. Furthermore, iminodiacetic acid was immobilized on the modified agarose. Finally, the derivatization efficiency was studied by measuring the epoxide groups and retention of Cu2+. The results indicated that the matrices were modified efficiently and the use of Na BH4 in small amounts is enough to provide a reducing environment to the reaction.