Rodney Smith 
Cell Technology for Cell Products [PDF ebook] 
Proceedings of the 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005

Contents; 19th ESACT Meeting Committees; ESACT Executive Committee; Sponsors; Companies in Trade Exhibition; Acknowledgements; List of Participants; CHAPTER I: PERSPECTIVES OF THE BIOTECH INDUSTRY; The UK bioprocessing sector, Tony Bradshaw; CHAPTER II: TRANSCRIPTION TO SECRETION; A Novel AIR-induced Gene Expression System in HEK.EBNA Cells and its Applications, N.S. Werner et al.; Towards Stronger Gene Expression – a Promoter’s Tale, S. Kalwy et al.; Improving the Expression of a Soluble Receptor: Fc fusion Protein in CHO Cells by Coexpression with the Receptor Ligand, G.W. Lee et al.; Growth, Apoptosis and Functional Genomics Analysis of CHO-K1 Over-Expressing Telomerase, F. Crea et al.; Microarray Analysis of Metabolically Engineered NS0 Cell Lines Producing Chimeric Antibody, G. Khoo et al.; Optimizing Medium for Transient Transfection, Z.-H. Geng et al.; Establishment of Recombinant CHO Cell Lines Under Serum-free Conditions, M. Kuchenbecker et al.; Novel Cell-based Assay for the Detection of Murine type I If N, M. Bollati-Fogolín et al.; Characterisation of the Induction of Cytochrome p450 Enzymes in Primary Cultures of Human Hepatocytes, T.L. Freeman et al.; Effect of Culture Conditions on Glycosylation of Recombinant beta-Interferon in CHO Cells, M. Spearman et al.; Induction of Caspase-3-dependent Apoptosis by Electrolyzed Reduced Water/platinum Nanoparticles in Cancer Cells, T. Hamasaki et al.; Molecular Mechanism of TAK1-Induced Repression of h TERT Transcription, M. Maura, et al.; Analysis of Genetic Parameters in Order to Get More Information on High Producing Recombinant CHO Cell Lines, C. Lattenmayer et al.; Redox Regulation by Reduced Waters as Active Hydrogen Donors and Intracellular ROS Scavengers for Prevention of type 2 Diabetes, S. Shirahata et al.; Transcription Profiling of Different Recombinant CHO Clones Based on Cross-species Microarray Analysis, E. Trummer et al.; Adaptation without Cell Division? K.L. Astley et al.; Release of Plasmid-DNC from PEI/DNA Particles, M. Bertschinger et al.; Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody, S. Matsumoto et al.; Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody, C.A. Caldas et al.; Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1, D.L. Hacker et al.; The Role of the Sumoylation Pathway in Transient and Stable Recombinant Protein Expression in Chinese Hamster Ovary Cells, M. Bertschinger et al.; Effect of Different Materials Used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium, P. Aizawa et al.; Antibody Production by GS-CHO Cell Lines Over Extended Culture Periods, A.J. Porter et al.; Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells, M. Schomberg et al.; Effect of the Scaffold in Maintenance of Suitable Chondrcyte Morphology in Cartilage Regeneration, J. Rodríguez et al.; Metabolic Changes Occuring During Adaptation to Serum Free Media, S. Denby et al.; The Expression of the neo gene in GS-NS0 Cells Increases their Proliferative Capacity, F. Veraitch et al.; CHAPTER III: THERAPEUTIC CELL ENGINEERING; The Therapeutic Potential of Human Embryonic Stem Cells, S.H. Cedar et al.; Investigations of Murine Embryonic Stem Cell Maintenance by Analyses of Culture Variables and Gene Expression, J.M. Piret et al.; Generation of Cardiomyocytes Derived from Mouse ESC in a Scale-up System, S. Niebruegge et al.; Expansion of Undifferentiated Human Embryonic Stem Cells, A.B.H. Choo et al.; New Electrofusion Devices for the Improved Generation of Dendritic Cell-tumour Cell Hybrids, J. Schaper et al.; Influence of c-Myc and bcl-2 CO-expression on Proliferation, Apoptosis and Survival in CHO Cultures, V. Ifandi et al.; Ex Vivo Expansion of Hematopoietic Stem Cells Using Defined Culture Media, J. Hartshorn et al.; Development of A Novel Serum-free Cryopreservative Solution, T. Toyosawa et al.; Hepatic Cell Lines Culture D on Different Scaffolds and in Different Stages for Bioartificial Liver Systems, K. Kaito et al.; Comparison of Flask and Bag Systems in Cultivation of Dendritic Cells for Clinical Application, L. Macke et al.; T-Lymphocytes Transduced with SFCMM-3 Vector in a Closed System, C. Benati et al.; Influence of Down-regulation of Caspase-3 and CASPASE-7 by Sirnas on Sodium Butyrate-induced Apoptotic Cell Death of Chinese Hamster Ovary Cells Producing Thrombopoietin, Y.H. Sung et al.; CHAPTER IV: GENE MEDICINE; UTRtech™: Exploiting m RNA Targeting To Increase Protein Secretion From Mammalian Cells, B. Stern et al.; Semi-Synthetic Mammalian Gene Regulatory Networks, B.P. Kramer et al.; Acoustic Cell Processing for Viral Transduction or Bioreactor Cell Retention, V.M. Gorenflo et al.; Comparison of Different Transient Retroviral Transfection Systems, V. Kleff et al.; CHAPTER V: CELLS TO TISSUES; Artificial Human Lymphnode, C. Giese et al.; Single Cell Study in a Hydrogel, T. Braschler et al.; Predicting Tissue Assembly of Prostate Cancer Spheroids, K. O’Connor et al.; Cultivating Cells of Different Origin for 3d Bone Constructs Considering Physiological Conditions, K. Suck et al.; Guidance on Good Cell Culture Practice, S. Coecke et al.; In Vitro Vascularization of Human Microtissues, J.M. Kelm et al.; Preparation of Salmon Atelocollagen Fibrillar Gel and Its Application to the Cell Culture, N. Nagai et al.; Propagation of USSC for Therapeutic Approaches, J. Lamlé et al.; Bioreactor Based Production of Functional Conditioned Medium for the Propagation of Undifferentiated Human Embryonic Stem Cells, A. Bauwens et al.; Large-scale Expansion of Cells for Use as Therapeutic Agents, J. Melero-Martin et al.; EX VIVO Expansion of CD34+ Cells in Stemline. I I Hematopoietic Stem Cell Expansion Medium Generates a Large Population of Functional Early and Late Progenitor Cells, S.L. Leugers et al.; In Vitro Bone Formation of Multipotential Human Mesenchymal Cells in Fixed Bed Perfusion Bioreactors, N. Zghoul et al.; Influence of Basal Media Formulations and Calcium on Growth and Directed Differentiation of Human Osteogenic Cells In Vitro, I. Majore et al.; CHAPTER VI: PROTEIN PRODUCTS; BHRF-1 as a Tool for Genetic Inhibition of Apoptotis in Hybridoma Cell Cultures, S. Juanola et al.; Karyotype of CHO DG44 cells, D. Martinet et al.; From Biopsy to Cartilage-carrier-constructs by Using Microcarrier Cultures as Sub-process, S. Nagel-Heyer et al.; Effect of Peptones and Study of Feeding Strategies in a CHO Based Fed-batch Process for the Production of a Human Monoclonal Antibody, V. Chotteau et al.; Retinoic Acid Induced Up-regulation of Monoclonal Antibody Production, Y. Inoue et al.; Media and Cell Manipulation Approaches to the Reduction of Adaptation Time in GS NS0 Cell Line, P. Clee et al.; The Production of Human Growth Hormone, F. Anton et al.; Preparation of Membrane Receptor Proteins Utilising Large Scale Micro-carrier Culture and a ‘Pearl Freezing Factory’ to Finalise the Product, I. Jansson et al.; Silk Protein Sericin Improves Mammalian Cell Culture, S. Terada et al.; Design of Culture Media for Drosophila Melanogaster S2 Cells Producing Recombinant G Glycoprotein from Rabies Virus, A.L.L. Galesi et al.; Comparison of the Cultivation of Wild and Transfected Drosophila Melanogaster S2 Cells in Different Media, K. Swiech et al.; Low Temperature Culture Effects on s CR1 Productivity by r CHO Cells, H. Matsuoka et al.; Proteomic Characterization of Recombinant NS0 Cell Clones Obtained by an Integrated Selection Strategy, A. Castillo et al.; IFN-Glycosylation Macroheterogeneity and Intracellular Nucleotide and Sugar Nucleotide Contents of CHO Cells Cultivated in Batch Culture, N. Kochanowski et al.; Influence of the Adaptation Procedure and the Serum-free Conditions on the N-glycosylation Pattern of EPO Produced by CHO Cells, F. Le Floch et al.; Stability and Cytogenetic Characterization of Recombinant CHO Cell Lines Established by Microinjection and Calcium Phosphate Transfection, M. Derouazi et al.; Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody, S. Matsumoto et al.; Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody, C.A. Caldas et al.; Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1, D.L. Hacker et al.; Effect of Different Materials used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium, P. Aizawa et al.; Antibody Production by GS-CHO Cell Lines Over Extended Culture Periods, A.J. Porter et al.; Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells, M. Schomberg et al.; Effect of the Scaffold in Maintenance of Suitable Chondrcyte Morphology in Cartilage Regeneration, J. Rodríguez et al.; Metabolic Changes Occuring During Adaptation to Serum Free Media, S. Denby et al.; The Expression of the neo Gene in GS-NS0 Cells Increases Their Proliferative Capacity, F. Veraitch et al.; 1000 Non-instrumented Bioreactors in a Week, M. Stettler et al.; Optimisation of Time-space yield for Hybridoma fed-batch Cultures with an Adaptive Olfo-controller, R. Pörtner et al.; Influenza Vaccines – Challenges in Mammalian Cell Culture Technology, Y. Genzel et al.; Towards p CO2 -optimised Fermentations – Reliable Sampling, p CO2 Control & Cellular Metabolism, A. Jockwer et al.; Cultivation of E-FL in Perfusion Mode Using Wave® Bioreactor and Stirred-Tank Reactor for Parvovirus Vaccine Production, B. Hundt et al.; Repeated Batch Fermentation Using a Viafuge Centrifuge, K. Russ et al.; Serum-free Transient Expression of Antibodies Using 40 Tray Cell Factories, P. Schlenke et al.; Monitoring Cell Physiologyin Influenza Vaccine Production by Flow Cytometry, J. Schulze-Horsel et al.; Nucleo Counter – An Efficient Technique for the Determination of Cell Number and Viability in Animal Cell Culture Processes, D. Shah et al.; On line Biomass Monitoring in Cell Culture with Scanning Radio-frequency Impedance Spectroscopy, J.P. Carvell et al.; The Use of a Mobile Robot for Complete Sample Management in a Cell Culture Pilot Plant, M. Wojtczyk et al.; Closed Loop Control of Perfusion Systems in High-density Cell Culture, A. Bock et al.; Optimization of Culture Medium with the Use of Protein Hydrolysates, P. Ganglberger et al.; Optimization of a Fed-batch Strategy for Production of Mab Ign 311 in Small Scale Experiments, P. Hinterleitner et al.; Serum-free Influenza Vaccine Production with MDCK Cells in Wave-bioreactor and 5L-stirred Tank Bioreactor, Y. Genzel et al.; Rotary Bioreactor for Recombinant Protein Production, S. Navran; Flow Cytometry as a Tool for Process Monitoring of Virus-cell Systems, K.S. Sandhu et al.; The Use of Multi-parameter Flow Cytometry for Characterisation of Insect Cell-baculovirus Cultures, B. Isailovic et al.; Understanding Metabolic Needs of EB14 Cells in Culture, J. Hartshorn et al.; Virus Production in Vero Cells Using a Serum-free Medium, K. Kolell et al.; Computational Fluid Dynamics Applied to Laboratory Scale Cell Culture Reactors for the Prediction of Hydrodynamic Stress, E. Olmos et al.; Using Genomic Tools for the Identification of Important Signaling Pathways in Order to Facilitate Cell Culture Medium Development, D.W. Allison et al.; Development and Application of An Animal-component Free Single-cell Cloning Medium for Chinese Hamster Ovary Cell Lines, N. Lin et al.; Plant Peptones: Nutritional Properties Sustaining Recombinant Protein Secretion by CHO Cells, J. Mols et al.; Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture, J.B. Ritter et al.; A Comparison of the Six Fors Fermenter System and Lab Scale Fermenters For Clone Selection, G. Wilson et al.; Comparison of Cultivations of CHO Cell Line in Chemostat and Acceleratostat Experiments, R.B. Nielsen et al.; Evaluation of a Novel Micro-bioreactor System for Cell Culture Optimisation, G. Wilson.; Comparison of Different Design Configurations of a 1-L Multi-bioreactor Station for Cell Culture Process Development, H. Schütte et al.; The Effect of Genetically Modified Insulin Growth Factor on Cell Proliferation and Adenovirus Productivity in Serum Free Medium, A. Buckler et al.; Evaluation of a Scalable Disposable Bioreactor System for Manufacturing of Mammalian Cell Based Biopharmaceuticals, S. Langhammer et al.; Purification of Rabies Virus Produced on Vero Cells Using Chromatography Techniques, M. Chtioui et al.; Human Growth Hormone (HGH) Purification From CHO-Cell Culture Supernatant Utilizing Macroporous Chromatographic Media, J.-G. Walter et al.; Process Development for a Veterinary Vaccine Against Heartwater Using Stirred Tanks, I. Marcelino et al.; Working Towards a Chemically-defined Replacement for Hydrolysates, Z.W. Deeds et al.; Effect of Vitamins or Lipids Addition on Adenovirus Production at High Cell Densities, T.B. Ferreira et al.; Observation and Analysis of Lab Scaled Microcarrier Cultivation by In-situ Microscopy with Image Processing Tools, G. Rudolph et al.; Rapeseed Peptidic Fractions as Medium Additives in Animal Cell Bioreactors, B. Farges et al.; Influence of Rapeseed Protein Hydrolysis Conditions on Animal Cell Growth in Serum-free Media Supplemented with Hydrolysates, G. Chabanon et al.; Rapid Media and Process Optimization Using DOE: A Case Study, S. Holdread et al.; Developing Chemically Defined Media Through DOE: Complete Optimization with Increased Protein Production in Less than 8 Months, K. Hammett et al.; Bioreactor Process Strategies for r NS0 Cells, D. Fernandez et al.; The Efficient Induction of Human Antibody Production in in Vitro Immunization by CPG Oilgodeoxynucleotides, Y.Aiba et al.; Rapid Culture Media Optimization to Gain Multiple Fold Increase in Recombinant Protein Production in Two CHO Cell Lines, S.D. Storms et al.; Strategies for Large Scale Production of Parapoxvirus Ovis NZ-2, M. Pohlscheidt et al.; Proteomic Investigation of Recombinant CHO Cells in High Density Culture, J.R. Cresswell et al.; Comparative Proteomic Study of Mechanisms Involved in the Expression of Recombinant Monoclonal Antibody in Non Amplified NS0 Myeloma Cell Line, L.E. Rojas et al.; Monitoring Nutrient Limitations by Online Capacitance Measurements in Batch & Fed-batch CHO Fermentations, S. Ansorge et al.; Characterisation of Shake Flasks for Cultivation of Animal Cell Cultures, G. Jänicke et al.; Manufacturing of Lots of Human T-Lymphocytes for Cell Therapy, N.D. Bernardi et al.; Oxygen Control in Static Cell Cultures, N.D. Bernardi et al.; The Effects of Cell Separation with Hydrocyclones on the Viability of CHO Cells, R.C.V. Pinto et al.; Erythropoietin Purification Employing Affinity Membrane Adsorbers, M.C.M. Mellado et al.; Cultivation of Vero Cells on Microporous and Macroporous Microcarriers, M.C.O. Souza et al.; CFD-Aided Design of Hollow Fibre Modules for Integrated Mammalian Cell Retention and Product Purification, R.L. Machado et al.; Comparison of Manufacture Technologies for RAV12 Monoclonal Antibody in Production Medium Containing No Animal Derived Proteins, I. Shackel et al.; Highly Efficient Serum-free Production of Biopharmaceuticals in CHO-Cells, S. Zahn et al.; A New Host Cell-line for the Fast, Reproducible and Efficient Production of Biopharmaceuticals, B. Greulich et al.; Proteomic Characterization of Recombinant NS0 Cell Clones Obtained by an Integrated Selection Strategy, A. Castillo et al.; Analysis and Modeling of the Metabolism of Lepidopteran Cell Lines, J.-C. Drugmand et al.; Growth of Mammalian and Lepidopteran Cells on Bio NOC® II Disks, a Novel Macroporous Microcarrier, J.-C. Drugmand et al.; Screening Strategiesfor Iron Chelators in Serum Free Media, J. Keenan et al.; Production of Human Growth Hormone in a Mammalian Cell High Density Perfusion Process, T. Nottorf et al.; Development of an Integrated Qualitative and Quantitative Approach to Study Expression-Secretion Bottlenecks of Mammalian c DNAs in HEK293 EBNA Cells, L. Glez et al.; Rotating Bed Bioreactors: a Novel Way to Natural Proteins by Tissue-like Cultivation of Mammalian Cells, H.P.A. Hoffmeister et al.; Minireactors for Animal Cell Culture: Monitoring of Cell Concentration and Cellular Activity, A. Soley et al.; Air-lift Bioreactor Improvement of Scale-up Linearity Between Shake Flasks and 25-L Scale in the Insect Cells–baculovirus Expression System, F. Bollin et al.; Name Index; Subject Index.