This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in ‘Proteomics’ should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.
Tabella dei contenuti
Sample Preparation;
How to Couple and Handle HPLC and Mass Spectrometry;
LC-MS of Proteins;
LC-MS of Membrane Proteins;
LC-MS of Hydrolyzed Proteins;
Online-digest-LC-MS of Proteins;
Quantitative LC-MS of Proteins;
Top-Down Analysis;
LC-MS for the Identification of posttranslational modifications on proteins;
Functional Analysis of Proteins by LC-MS;
Bioinformatical Tools for the LC-MS Analysis of Proteins;
Industrial Standards and Strategies in LC-MS Analysis of Proteins
Circa l’autore
Dr Thomas Letzel is Head of Analytical Research at Munich Technical University.
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