This volume assembles a broad spectrum of methods used in long non-coding RNAs (lnc RNA) research, ranging from computational annotation of lnc RNA genes to molecular and cellular analyses of the function of individual lnc RNA. Long Non-Coding RNAs: Methods and Protocols also discusses methods used to study circular RNAs and RNA splicing, as well as influential findings on lnc RNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Long Non-Coding RNAs: Methods and Protocols is a must-have for molecular biologists, cell and developmental biologists, specialists who conduct disease-oriented research, and bioinformatics experts who seek a better understanding on lnc RNA expression and function by computational analysis of the massive sequencing data that are rapidly accumulating in recent years.
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