This book constitutes the proceedings of the 18th International Conference on Practical Applications of Agents and Multi-Agent Systems, PAAMS 2020, held in L’Aquila, Italy, in October 2020.The 29 regular and 17 demo papers presented in this volume were carefully reviewed and selected from 64 submissions. They deal with the application and validation of agent-based models, methods, and technologies in a number of key applications areas, including: advanced models and learning, agent-based programming, decision-making, educa-tion and social interactions, formal and theoretic models, health and safety, mobility and the city, swarms and task allocation.