Optogenetic tools have allowed significant advances in the understanding of biological problems, particularly in the neurosciences field. Biological tools as well as optical set-ups have evolved and a wide range of probes and light-controllable modules are now available.
The aim of this book is to give a flavour of illumination strategies and imaging with an overview of the different optogenetic tools and their main applications in cell biology. Based on examples covering the different aspects of cell biology, this book provides a practical approach for using light-emitting sensors and light-driven actuators.
Inhoudsopgave
Fast Volumetric Imaging Using Light-sheet Microscopy – Principle and Applications;
Super-Resolution Microscopy;
The Glowing Panoply of Fluorogen-based Markers for Advanced Bioimaging;
Optogenetic Reporters for Cell Biology and Neuroscience;
Spatiotemporal Dynamics of Cellular Signaling Processes;
Optogenetic Control of Generation of Reactive Oxygen Species for Photo-inducible Protein Inactivation and Cell Ablation;
Optogenetic Tools for Quantitative Biology: The genetically-encoded Phy B-PIF Light-inducible Dimerization System and its Application for Controlling Signal Transduction;
Quantitative Control of Kinase Activity with a Mathematical Model;
Light control of Transcription in Cells;
Building a Light-Inducible Receptor Tyrosine Kinases;
Mechanotransduction and Optogenetics
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