The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes.
This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation.
Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
Inhoudsopgave
The Photoproteins (by 2008 Nobel Laureate Osamu Shimomura)
Luminous Marine Organisms
Beetle Luciferases: Colorful Lights on Biological Processes and Diseases
Split Luciferase Systems for Detecting Protein-Protein Interactions in Mammalian Cells
Photoproteins in Nucleic Acid Analysis
Bioluminescence Resonance Energy Transfer in Bioanalysis
Photoproteins as in Vivo Indicators of Biological Function
Photoproteins as Reporters in Whole-cell Sensing
Luminescent Proteins in Binding Assays
Applications in Microfluidics and Miniaturized Analytical Systems
Advances in Instrumentation for Detecting Low-level Bioluminescence and Fluorescence
Photoproteins and Instrumentation: Their Availability and Applications in Bioanalysis
Over de auteur
Sylvia Daunert is the Gill Eminent Professor of Analytical and Biological Chemistry and Professor of Pharmaceutical Sciences at the University of Kentucky. Her research interests lie in the area of bioanalytical chemistry, at the interface between analytical chemistry, molecular biology, and bioengineering. Dr. Daunert is the author or co-author of over 100 original research publications and of several patents in the area of bioanalytical chemistry. Her group’s research has also been featured in textbooks, scientific journals, as well as in the press all over the world.
Sapna K. Deo is Assistant Professor at the Department of Chemistry and Chemical Biology, Indiana University Purdue University Indianapolis. She gained her Ph D in bioanalytical chemistry from the University of Kentucky in 2000, where she also held the posts of postdoctoral fellow and assistant research professor. Dr. Deo has co-organized a symposium for the American Chemical Society, of which she is a member. She is a reviewer for several journals in the field.
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