In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface c DNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
Spis treści
Part I: Yeast Surface Antibody Display Library and Antibody Engineering
1: Protein Engineering and Selection Using Yeast Surface Display
Alessandro Angelini, Tiffany F. Chen, Seymour de Picciotto, Nicole J. Yang, Alice Tzeng, Michael S. Santos, James A. Van Deventer, Michael W. Traxlmayr, and K. Dane Wittrup
2: Isolation and Validation of Anti-B7-H4 sc Fvs from an Ovarian Cancer sc Fv yeast-Display Library
Denarda Dangaj and Nathalie Scholler
3: Combining Phage and Yeast Cell Surface Antibody Display to Identify Novel Cell Type-Selective Internalizing Human Monoclonal Antibodies
Scott Bidlingmaier, Yang Su and Bin Liu
4: Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates
Benjamin J. Tillotson, Jason M. Lajoie and Eric V. Shusta
Part II: Yeast Surface Display as a Tool For Protein Engineering
5: Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast
Li Yi, Joseph M. Taft, Qing Li, Mark C. Gebhard, George Georgiou, Brent L. Iverson
6: T Cell Receptor Engineering and Analysis Using the Yeast Display Platform
Sheena N. Smith, Daniel T. Harris, and David M. Kranz
7: Epitope Specific Binder Design by Yeast Surface Display
Jasdeep K. Mann and Sheldon Park
8: Applications of Yeast Surface Display for Protein Engineering
Gerald M. Cherf and Jennifer R. Cochran
PART III: Yeast Surface c DNA Display Library Construction and Applications
9: Identification of Novel Protein-Ligand Interactions by Exon Microarray Analysis of Yeast Surface Displayed c DNA Library Selection Outputs
Scott Bidlingmaier and Bin Liu
10: Identification of Post-Translational Modification-Dependent Protein Interactions using Yeast Surface Displayed Human Proteome Libraries
Scott Bidlingmaier and Bin Liu
11: Utilizing Yeast Surface Human Proteome Display Libraries to Identify Small Molecule-Protein Interactions
Scott Bidlingmaier and Bin Liu
Part IV: Yeast Surface Display in Bioassay and Industrial Applications
12: Enzyme Evolution by Yeast Cell Surface Engineering
Natsuko Miura, Kouichi Kuroda, and Mitsuyoshi Ueda
13: Electrochemical Glucose Biosensor Based on Glucose Oxidase Displayed on Yeast Surface
Hongwei Wang, Qiaolin Lang, Bo Liang, and Aihua Liu
14: Couple Binding to Catalysis – Use Yeast Cell Surface Display to Select Enzymatic Activities
Keya Zhang, Karan Bhuripanyo, Yiyang Wang, and Jun Yin
15: The use of Yeast Surface Display in Biofuel Cells
Alon Szczupak and Lital Alfonta
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