Sandy B. Primrose & Richard Twyman 
Principles of Gene Manipulation and Genomics [PDF ebook] 

Preface.
1. Gene Manipulation in the Post-Genomics Era.
Part I: Fundamental Techniques of Gene Manipulation:.
2. Basic Techniques.
3. Cutting and Joining DNA Molecules.
4. Basic Biology of Plasmid and Phage Vectors.
5. Cosmids, Phasmids and Other Advanced Vectors.
6. Gene Cloning Strategies.
7. Sequencing Genes and Short Stretches of DNA.
8. Changing Genes: Site-directed Mutagenesis and Protein
Engineering.
9. Bioinformatics.
Part II: Manipulating DNA in Microbes, Plants and
Animals:.
10. Cloning in Bacteria Other Than Escherichia coli.
11. Cloning in Saccharomyces cerevisiaea and Other
Fungi.
12. Gene Transfer to Animal Cells.
13. Genetic Manipulation of Animals.
14. Gene Transfer to Plants.
15. Advanced Transgenic Technology.
Part III: Genome Analysis, Genomics and Beyond:.
16. The Organization and Structure of Genomes.
17. Mapping and Sequencing Genomes.
18. Comparative Genomics.
19. Large-Scale Mutagenesis and Interference.
20. Analysis of the Transcriptome.
21. Proteomics I – Expression Analysis and
Characterization of Proteins.
22. Proteomics II – Analysis of Protein Structures.
23. Proteomics III – Protein Interactions.
24. Metabolomics and Global Biochemical Networks.
Part IV: Applications of Gene Manipulation and
Genomics:.
25. Applications of Genomics: Understanding the Basis of
Polygenic Disorders and Identifying Quantitative Trait Loci.
26. Applications of Recombinant DNA Technology.
Index

Sandy Primrose was an academic for 10 years and a senior
manager in the biotechnology industry for 20 years. He now manages
a consultancy practice focused on technology management.
Richard Twyman worked as a research scientist at the
University of Warwick and at the MRC Laboratory of Molecular
Biology in Cambridge before taking up scientific writing as a full
time occupation in 1998. He is currently a Visiting Fellow at the
Department of Biology, University of York, and a freelance
scientific writer and teacher.