In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives.
Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.
Spis treści
In-cell NMR Spectroscopy: From 2001 to Now; Protein NMR in Escherichia coli; In-cell NMR in Eukaryotic Cells – I (Introduction from Outside of Cells); In-cell NMR in Eukaryotic Cells – II (Direct Protein Expression); Protein Structure Determination in Living Cells from NOE-derived Distance Restraints; In-cell Structure Determination Using Paramagnetic NMR Spectroscopy; Bioreactor System for In-cell NMR Observation; 19F In-cell NMR; Cellular Solid-state NMR Spectroscopy; In-cell EPR; In-cell NMR: Spectral Optimization and Population Adjustment by Rational Protein Engineering; Protein Stability and Weak Intracellular Interactions; Protein Folding, Maturation and Redox State; All-atom Molecular Dynamics Simulation of Proteins in Crowded Environments; Primary Drug Screening by In-cell NMR Spectroscopy; In-cell NMR Spectroscopy of Nucleic Acids
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