Francesc Gòdia & Martin M. Fussenegger 
Animal Cell Technology Meets Genomics [PDF ebook] 
Proceedings of the 18th ESACT Meeting. Granada, Spain, May 11-14, 2003

Meeting Committees; ESACT Executive Committee; Sponsors; Companies Participating in the Trade Exhibition; Introduction: Animal Cell Technology Meets Genomics; Acknowledgements; List of Participants; CHAPTER 1: CELLULAR MECHANISMS; Effect of osmotic pressure on GS-NSO expression system, Wu M., et at.; Molecular approaches to influence epigenetic effectors of transient and stable transgene expression in mammalian cells, Hacker D. et al.; Genomic exploration on Chinese hamster ovary cells, de Leon Gatti M. et al.; Roles for glycosylation in receptor-ligand interactions in the immune system, Rudd, P.M. et al.; Investigations on mannose-6-phosphate receptor mediated protein uptake, Duerrschmid M., Jursik, et al.; Analysis of multiple apoptosis parameters using a microfluidic chip-based system, Valer M. et al.; Inhibiting apoptosis in cell culture using multiple inhibitors, Figueroa, B. et al.; Pausing of mammalian cells by cold exposure, limits and Opportunities, Hunt L. et al.; The functional competence of animal cells: analysis of the secretory pathway, Racher A.J. et al.; Proteome analysis of recombinant CHO cells under hyperosmotic stress, Lee M.S. et al.; Expression profiling analysis of sodium butyrate-induced Chinese hamster ovary cells in defined medium, Melville M.W. et al.; Intracellular nucleotide pools for optimizing productoriented transient transfection of HEK293 cells in suspension, Bassani Molinas M.M. et al.; Impact of yeast pyruvate carboxylase on the productivity of animal host cell lines, Bollati Fogolín M. et al.; Environmental effects of lactate on High-Five™ insect cell Metabolism, Drugmand J.-C. et al.; Correlation of intracellular nucleotide pools to amino acid concentrations in culture media by the application of multivariate methods, Hesse F. et al.; Engineering Trichoplusia ni (High Five™) insect cells to express a cytosolic pyruvate carboxlase enzyme improves the viability of the cells in batch, fed-batch and perfusion cultures, Locas M.-C. et al.; Insect cell culture medium selection and optimisation based on monitoring and economical considerations, Lecina M. et al.; Antiapoptotic activity of synthetic and natural peptides, Franek F. et al.; Effect of antiapoptotic genes expression on cell growth and monoclonal antibody productivity in a hybridoma cell line, Juanola S. et al.; Amplified dicistronic expression units mediate apoptosis protection in CHO-DG44 cells adapted for growth in serum-free media, impact on mitochondria copy number, Meents H. et al.; Effect of reduced water on the apoptotic cell death triggered by oxidative stress in pancreatic ß HIT-T15 cell, Shirahata S. et al.; Characterization of apoptosis in a CHO cell line cultivated in batch and continuous culture: Effect of medium, specific perfusion rate and chemical inhibitors, Thrift J. et al.; Hydrogen peroxide-induced cellular senescence is regulated via two different pathways, Yoshizaki K. et al.; Identification of autocrine factors influencing proliferation in serum free cultures of Trichoplusia ni cells, Eriksson U. et al.; The effects of insulin and Long™R3IGF-I on Chinese hamster ovary cells: cell survival, receptor activation and second messenger pathways. Yandell C.A. et al.; Conditioned medium factors in protein-free cultures of NS0 cells, Spens E. et al.; Production of human anti-peptide antibody for clinical use by in vitro immunization, Tamura, T. et al.; Optimization of in vitro immunization protocol to produce antigen specific human monoclonal antibody by demonstrating the role of IL-10, Xu Q.H. et al.; Extracellular histone H4 from SF9 cells is antimicrobial, Calles K. et al.; Analyzing calcium phosphate transfection of adherent CHO cells using microscopic live imaging, Grosjean F. et al.; Effects of multiplicity of infection (MOI) and cell cycle on Baculovirus infection kinetics, Haas R. et al.; Non-freezing preservation and subsequent recovery of hepatocytes under p O2- and p H-controlled conditions forcontinuous supply of cells for bioartificial liver support, Iding K. et al.; Selection of MDCK subclones with various genotypic and phenotypic markers: sensitivity to influenza virus infection, Kessler N. et al.; Extracellular metalloproteases and their inhibitors expressed by the IFN-ã producing CHO-320 cell line, Mols J. et al.; Novel small-molecule AKT1 inhibitors discovered by Redistribution™-based high-throughput screening. Præstegaard M. et al.; Influence of mistletoe extracts and its components on invitro physiology of cancer cells, Sidler F. et al.; Rapid in-process monitoring of antibody integrity, purity and titre-2100 Bioanalyser (Lab on a chip) study, Nesredin A. et al.; Toxicological evaluation of haine river waste-water samples to appropriately sensitised cultured Fathead Minnow cells compared with the microtox® method, Dierickx P.J. et al.; CHAPTER 2: CELL BASED THERAPIES; 3D-cultivation and characterisation of osteogenic cells for the production of highly viable bone tissue implants, Barthold M. et al.; Carbon source has a considerable influence on extracellular matrix formation, demonstrated on specific collagen expression of primary human tenocytes, Möbest D. et al.; Design and characterization of cardiomyocyte-derived micro issues, Kelm M.J. et al.; Process development for standardized generation of monocyte-derived dendritic cells: applicability in breast cancer immunotherapy, Bohnenkamp H.R. et al.; Stemline™ hematopoietic stem cell expansion medium, a serum-free medium for expansion of CD34+ hematopoietic stem cells and progenitors, Leugers S. et al.; Mesenchymal stem cells: a very first step towards large scale cell culture strategies transplantation, Bensellam M. et al.; Expansion of murine embryonic stem cells on microcarriers-functional characteristics and scale-up potential, Burg M. et al.; In vitro cultivation of rabbit mesenchymal stromal cells on 3D bioresorbable calcium phosphate scaffolds for the generation of bone tissueimplants, Fargali S. et al.; Establishment and characterization of human medullary thyroid carcinoma cell lines for immunotherapy, Stradler G. et al.; Suppression of cell growth by platinum nanocolloids as scavengers against reactive oxygen species, Hamasaki T. et al.; Comparison of fluidized bed and fed batch reactor cultures for production of anti-HIV-antibody, Hinterleitner P. et al.; Suppression of oxidative stress-induced apoptosis of neuronal cells by electrolyzed-reduced water, Kashiwagi T. et al.; Spheroid formation by encapsulation of cancer cells to mimic small size tumors, Marc A. et al.; Bioreactor for continuous biomechanical characterization of cellular systems and tissue-engineered biohybrid tissues, Möbest D. et al.; Membrane-separated cocultivation of cord blood hematopoietic stem cells with stromal cell lines, Fischbach T. et al.; Anti-apoptotic genes protect an insulinoma cell line from cell death induced by hyperglycemia, Ogawa A. et al.; Preparation and characterization of an idiotype-dendritic cell vaccine for immunotherapy of multiple myeloma, Opalka B. et al.; Pretreatment with polycations enhances Adenoviral infection levels in serum-free PER.C6™ cells as well as HEK 293 cells, Padilla J. et al.; Optimizing the production of cardiomyocytes from mouse embryonic stem cells in a 2L stirred tank reactor, Schroeder M. et al.; Establishment and characterization of CD4+ Killer-T-Cells (KTCs), Voglauer R. et al.; CHAPTER 3: GENE BASED THERAPEUTICS; Successful development of a robust adenovirus production process primarily relies on a better understanding of packaging cell line physiology and vector replication kinetics, Kamen A. et al.; Retroviral vector stability: inactivation kinetics and membrane properties, Cruz P. et al.; Comparison of host cell lines and production methods for a new generation of oncolytic adenoviral vectors, Forestell S. et al.; Process development for a veterinary vaccine against heartwater, Marcelino I. et al.; A newmethod for the quantitative determination of enveloped viral particles, Beer C. et al.; Development of a 293T cell line for the production of virus like particles in bioreactors, Costa M.J.L. et al.; Effect of MOI and medium composition on adenovirus infection kinetics, Ferreira T. et al.; ION-exchange chromatography based strategies for purification of viral vectors, Vasi J. et al.; Scaling up of endothelial cells culture for the production of a rickettsiae vaccine: evaluation of the importance of the interference effects, Hendrick V. et al.; CHAPTER 4: TARGET DISCOVERY; Inducible gene expression for antibiotic drug discovery and diagnostics Weber W. et al.; Nucleic acid capture assay: a novel high-throughput method for direct m RNA quantitation Van de Goor J. et al.; High throughput transient transfections of HEK293EBNA cells: development of an integrated expression purification process at 100 ml scale, Heine H., Rey L. et al.; Transient gene expression in suspension HEK293 cells: application to large-scale protein production, Baldi L., Jacquet R. et al.; Monoclonal antibody production: determination of appropriate system and medium for production enhancement and decrease of overall production time, Fritchman K. et al.; The secrets of transfection in serum-free suspension culture Geisse S. et al.; From genes to proteins: full open reading frame (ORF) clones and their application in functional research, Goedde A. et al.; A versatile disposable culture system for high throughput screening of process parameters and production cell lines, Jordan M. et al.; Long time storage of pre-inoculated multi-well plates for cell-based assay kits used for drug identification and characterization, Loa A. et al.; Comparative secretion levels of recombinant proteins between insect cell-baculovirus and mammalian HEK293EBNA cells, Losberger C. et al.; Microfluidic technology applied to protein sizing and Quantitation, Valer M. et al.; Cryo Stock – a software for cell culturemanagement, Zerlin M. et al.; CHAPTER 5: BIOPHARMACEUTICALS; Targeting the human Immunoglobulin loci for high level heterologous gene expression, Winkler K. et al.; MAR elements as tools to increase protein production by CHO cells, Girod, P.-A. et al.; Cell line engineering for production of therapeutic antibody glycoforms with increased biological activity, Umana P. et al.; Development of a metabolically optimized fermentation process based on glucose-limited CHO perfusion culture; Applications of quasi real-time metabolic flux analysis in mammalian cell culture process, Goudar C. et al.; Data-based modelling of cell cultures, Cunha-Bakeev C. et al.; Cell culture and downstream processing integration using Protein Chip® technology. Application to protein analysis and purification in biopharmaceutical manufacturing, Bengio S. et al.; The prospects for insect cells for use as a cell substrate for the production of biotherapeutics, Galbraith D.N.; High-performance cell culture platform technology for Mab production, Chang D.Y.H. et al.; Development and integration of a new animalcomponent-free process for the production of UK- 279, 276, Pluschkell S. et al.; Towards an affinity-based capture system for the isolation of high expression cells using a co-expressed surface protein, Derouazi M. et al.; Effect of doxycycline-regulated calnexin and calreticulin expression on specific thrombopoietin productivity of recombinant CHO cells, Chung J.Y. et al.; Recombinant glycoprotein expression. Development of a homologous CHO expression system, Noone C. et al.; Generation of recombinant antibody manufacturing cell lines using cell-cell fusion, Zeng W. et al.; Cloning and expression of biopharmaceuticals in CHO.K1 and BHK-21 cells, Castilho L.R. et al.; Camel single-domain antibodies as modular building blocks to make bivalent constructs for use in immunotheraphy of cancer, Cortez-Retamozo V. et al.; Establishment of efficient cloning method for variable region genes of antigenspecific human monoclonal antibody, Matsumoto S. et al.; From gene to monoclonal antibody: efficient screening by cell sorting, Borth N. et al.; Development of an integrated strategy for recombinant cell line selection, Castillo A.J. et al.; Gene transfer to animal cells in culture by microinjection: A novel tool to create recombinant cell lines? Derouazi M. et al.; Evaluation of the stability of NS0 cell lines expressing recombinant human Ig G, Forrest-Owen W. et al.; High throughput screening and selection of single cells in suspension using fluorescence parameters, Giese C. et al.; Control of key parameters in the development of mammalian production clones Kunert R. et al.; Establishment of screening systems for recombinant cell lines: early prediction of the suitability as a production clone, Lattenmayer C. et al.; Characterisation of CHO subclones showing profound changes in performance when propagated in different cultivation systems, Trummer E. et al.; High level production of recombinant Ig G in the human cell line PER.C6™, Yallop C. et al.; Creating a new medium to help meet the variable nutritional requirements of Chinese hamster ovary (CHO) cell clones, Deeds Z.W. et al.; Development of an efficient medium optimization kit for factorial matrix design – a statistical approach to increase cell growth and productivity of recombinant CHO cells, Delong B. et al.; An efficient approach to cell culture medium optimization – a statistical method to medium mixing, Gifford J. et al.; Virus inactivation in foetal calf serum by a combined, treatment of gamma-irradiation and UV-C irradiation, Hermann H. et al.; Advances in media optimization: two automated approaches that increase expression while reducing development time, Holdread S. et al.; The importance of cholesterol for insect cell growth and baculovirus production, Lua L.H.L. et al.; Use of rapeseed proteins and peptides as supplements in insect cell cultures, Marc A. et al.; Nutritional performance ofwheat gluten -derived protein hydrolysates in hybridoma cell cultures, Martens D.E. et al.; Synthe Chol™ synthetic cholesterol for cholesterol dependent cell culture. Development of non-animal derived chemically defined NS0 medium, Talley D. et al.; NS0 derivatives: MAb production in large-scale SFM formats, Manwaring J. et al.; Silk protein sericin accelerates proliferation of various mammalian cells, Terada S. et al.; Image analysis based realtime-control of glucose concentration, Brueckerhoff T. et al.; On line analysis of microcarrier cultivations, Burzlaff A. et al.; Error analysis during estimation of metabolic fluxes through metabolite balancing, Goudar C. et al.; Generalized logistic equation modeling of mammalian cell batch cultures, Goudar C. et al.; In-situ microscopy based monitoring of mammalian cell culture processes, Joeris K. et al.; A novel disposable microtube for rapid assessment of biomass in cell cultures, Jordan M.; Intracellular monitoring of baculovirus infection of Sf9 insect cells and r-protein production using anti-alpha-1, 3/4 fucosyltransferase antibodies and flow cytometry, Marc A. et al.; Effect of glucose and glutamine concentration on metabolism of animal cells in chemostat culture, Matsuoka H. et al.; Evaluation of a novel capacitance probe for on-line monitoring of viable cell densities in batch and fedbatch animal cell culture processes, Schmid G. et al.; Sodium butyrate enhancement of protein expression in He La cells: follow-up of a chimeric reporter gene behaviour for process development, Bassens C. et al.; Scalable, serum-free, high efficiency transfection of CHO Cells using an economical DNA transfer vehicle, Girard P. et al.; Process optimization for an NS0-derived hybridoma cell line in a chemically defined, protein-free medium, Johnson J.M. et al.; Impact of change in fermentation process p H and dissolved carbon dioxide concentration on secreted antibody structure and cell physiology of a GS-NS0 cell line expressing ahuman antibody, Ridley A. et al.; Experimental factorial design for the study of some variables in roller bottles cell culture, Rodríguez E.N. et al.; Evaluation of Roller Cell system for high scale production of biopharmaceuticals, Rodríguez E.N. et al.; Strategies for the process development of monoclonal antibodies for therapeutic use, De Mattei C. et al.; Optimisation of Pei-mediated transient expression in Chinese hamster ovary cells, Tait A. et al.; Development of fed-batch process producing monoclonal antibodies using in-house media, Huang Y.M. et al.; Production of an anti â2 integrin monoclonal antibody by a hybridoma cell line grown in a 2 liter bioreactor, Kallel H. et al.; Economic evaluation of disposables vs stainless steel within a modular pilot plant facility – a comparative analysis, Monge M. et al.; c GMP manufacturing of a fusion protein in mammalian cells using a large scale acoustic perfusion system, Crowley J. et al.; Influence of culture parameters on viability in perfusion process, Dalm M.C.F. et al.; Use of hydrocyclone as an efficient tool for cell retention in perfusion cultures, Elsayed E.A. et al.; Effect of different variables on the long-term spinfilter clogging during pilot-scale animal cell perfusion runs Figueredo A. et al.; Integrating acoustic perfusion in mammalian cell culture: temperature control Keijzer T. et al.; Flexible fed-batch process control of animal cells Frahm B. et al.; Development of a fed-batch strategy for the production of NS0-derived humanised MAb in protein free medium, Ojito Magaz et al.; Transfection to manufacturing: reducing timelines for high yielding GS-CHO processes, Rendall M. et al.; Optimization of a fed-batch process producing humanized antibodies, Sauer P.; Optimized feeding strategy for NS0 cells, Walowitz J. et al.; Comparison of complementary interactions between synthetic ligands and t-PA by HPMDC, Tappe A. et al.; Effect of nutrient supplementation on the biological quality of t-PAproduced by CHO cells on serum-free medium, Altamirano C. et al.; Modulation of the glycosylation repertoire of a recombinant human EPO expressing model cell line under different culture conditions, Duvar S. et al.; Physicochemical estimation of rh EPO potency produced in suspension culture, Etcheverrigaray M. et al.; Post-translational modification of recombinant human GM-CSF expressed by stable-transfected CHO cells under different culture conditions Forno Á.G. et al.; Softmix, a new scaleable mixing system for the cultivation of sensitive cells on microcarrier under protein-free conditions, Naschberger S. et al.; Establishment of fermentation process for hrepo using stirred tank fermenters in perfusion mode using a commercial protein-free culture medium, Bouzó L. et al.; Continuous GMP production in hollow fibre bioreactors-a viable alternative for the production of low volume biopharmaceuticals, Brecht R. et al.; Optimizing cultivation strategies in different scales of hollow fiber bioreactors, Rodríguez G. et al.; Increasing antibody production from hollow fiber systems, Gramer M.J. ;Production of poliovirus, rubella irus, measles virus and recombinant adenovirus in protein-free media, Card C. et al.; Serum-free production of poliovirus: a comparative study using microcarriers, roller bottles and stationary cell culture, Card C. et al.; Glutamine-free media for vaccine production processes, Genzel Y. et al.; Serum free cultivation of primary chicken embryo fibroblasts in microcarrier systems for vaccine production, Hundt B. et al.; Design of an animal protein free medium to sustain Vero cells growth in a stirred tank bioreactor, Kallel H. et al.; Use of on-line our measurements to monitor and improve insect cell cultures for recombinant protein production, Lecina M. et al.; Production of live influenza vaccine in MDCK cell line, Mazurkova N. et al.; Elaboration of the microencapsulated forms of the viral vaccines, Nechaeva E. et al.; HTS-based development ofhigh-producing CHO cell lines, Sautter K. et al.; Author Index; Subject Index.