Induced mutagenesis is a common and promising method for screening for new crops with improved properties. This title introduces the different methods and then focuses on the screening, detection and analysis of the novel mutations. Written by a global team of authors the book is an indispensable tool for all scientists working on crop breeding in industry and academia.
Cuprins
INDUCED MUTATIONS
Physically induced mutagenesis: Ion beam mutagenesis (Magori, Tanaka, Kawaguchi)
Ds transposon mutant lines for saturation mutagenesis of the Arabidopsis genome (Kuromori, Hirayama)
Use of mutants from T-DNA insertion populations generated by high-throughput screening (Stracke, Huep, Weisshaar)
Making mutations is an active process: Examining DNA polymerase errors (Eckert, Gestl)
Tnt1 induced mutations in Medicago: Characterisation and Applications (Ratet, Cosson, Wen, Tadege, Mysore)
MUTATION DISCOVERY
Sequencing by synthesis: Mutation detection with the Illumina Genome Analyzer (Lakdawalla, Schroth)
Chemical methods for mutation detection: The chemical cleavage of mismatch (CCM) method (Tabone, Sallmann, Cotton)
Mutation detection in plants by enzymatic mismatch cleavage (Till)
Mutation scanning and genotyping in plants by high resolution DNA melting (Mc Kinney, Nay, Koeyer, Reed, Wall, Palais, Jarret, Wittwer)
In silico methods: Mutation detection software for Sanger sequencing, fragment and genome analysis (Le Van*, Liu, Shouyong)
HIGH-THROUGHPUT SCREENING METHODS
Use of TILLING for reverse and forward genetics of rice (Terauchi)
Sequencing-based high-throughput mutation screening using Key Point TM technology (Rigola, van Eijk)
APPLICATIONS IN PLANT BREEDING
Natural and induced mutants of barley: SNPs in genes important for breeding (Thomas, Waugh, Forster, Franckowiak)
Association mapping for the exploration of genetic diversity and identification of useful loci for plant breeding (Belo, Luck)
Using mutations in corn breeding programs (Scott, Bodnar)
Gene targeting as a precise tool for plant mutagenesis (Zobell, Reiss)
EMERGING TECHNOLOGIES
Direct single-molecule DNA sequencing:Helicos Bio Sciences technology and applications (Jenkins, Kahvejian)
Mutation detection by genome sequencing with universal probes (Linnarsson)
Emerging Technologies: Detection of mutations with the Pinpoint Sequencer (Ju)
Emerging Technologies: Nanopore Sequencing for Mutation Detection (Rollings, Li)
Glossary
Despre autor
Gunter Kahl is Professor for Plant Molecular Biology in Frankfurt am Main, Germany. He has served in expert missions for IAEA, FAO and UNESCO across the world and is author of more than 250 scientific publications, including several Wiley-VCH books.
Khalid Meksem is Professor at the Department of Plant, Soil & General Agriculture of Southern Illinois University. He is associate editor of the Journal of Biomedicine and Biotechnology and chaired the Functional Genomic Workshop at the International Plant & Animal Genome Conference in San Diego. He is reviewer for several granting agencies, including the National Science Foundation and the US Department of Agriculture.
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