This book provides an overview of the research on production processes for bioethanol fuels in general, hydrolysis of the pretreated biomass for bioethanol production, microbial fermentation of hydrolysates and substrates with yeasts for bioethanol production, and separation and distillation of bioethanol fuels from the fermentation broth, complementing the research on biomass pretreatments presented in the first volume.It presents an overview of the research on biomass hydrolysis in general, wood hydrolysis, straw hydrolysis, and cellulose hydrolysis for bioethanol fuel production in the first section for biomass hydrolysis. It provides an overview of the research on microbial hydrolysate fermentation for bioethanol production in general, alternative fermentation processes for bioethanol fuel production such as simultaneous saccharification and fermentation (SSF) and consolidated biomass processing (CBP) compared with the separate hydrolysis and fermentation (SHF) process, metabolic engineering of microorganisms and substrates for bioethanol fuel production, and utilization of Saccharomyces cerevisiae for microbial fermentation of hydrolysates for bioethanol fuel production in the second section for hydrolysate fermentation.It provides an overview of the research on the bioethanol fuel separation from the fermentation broth in the last section. This book is a valuable resource for the stakeholders primarily in the research fields of energy and fuels, chemical engineering, environmental science and engineering, biotechnology, microbiology, chemistry, physics, mechanical engineering, agricultural sciences, food science and engineering, materials science, biochemistry, genetics, molecular biology, plant sciences, water resources, economics, business, management, transportations science and technology, ecology, public, environmental and occupational health, social sciences, toxicology, multidisciplinary sciences, and humanities among others.