Proteomics – the analysis of the whole set of proteins and their functions in a cell – is based on the revolutionary developments which have been achieved in protein analysis during the last years.
The number of finished genome projects is growing and in parallel there is a dramatically increasing need to identify the products of revealed genes. Acting on a micro level modern protein chemistry increases our understanding of biological events by elucidating the relevant structure-function relationships.
The second edition of the successful title Microcharacterization of Proteins presents a current overview of modern protein analysis: From sample preparation to sequence analysis, mass spectrometry and bioinformatics it informs about the tools needed in protein research. This makes the book indispensable for everyone involved in proteomics!
Cuprins
SECTION I: OVERVIEW
Microcharacterization of Proteins
SECTION II: MICROSEPARATION TECHNIQUES AND SAMPLE PREPARATION
Microseparation Techniques I: High Resolution Gel-Electrophoretic Techniques – Qualitative, Quantitative and Micropreparative Applications
Microseparation Techniques II: Gel Electrophoresis for Sample Preparation in Protein Chemistry
Microseparation Techniques III: Electroblotting
Microseparation Techniques IV: Analysis of Peptides and Proteins by Capillary Electrophoresis
Microseparation Techniques V: High Performance Liquid Chromatography
Sample Preparation I: Removal of Salts and Detergents
Sample Preparation II: Chemical and Enzymatic Fragmentation of Proteins
SECTION III: BIOANALYTICAL CHARACTERIZATION
Amino Acid Analysis
Chemical Methods for Protein Sequence Analysis
Analyzing Post-Translational Protein Modifications
Analysis of Biopolymers by Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
MALDI Postsource Decay Analysis
Electrospray Mass Spectrometry
Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS)
Sequence Analysis of Proteins and Peptides by Mass Spectrometry
SECTION IV: COMPUTER SEQUENCE ANALYSIS
Internet Resources for Protein Identification and Characterization
Protein Sequences and Genome Databases
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