This book summarizes current data on the architecture, structure-function relationships, reaction mechanisms and active site coupling in multienzyme complexes of 2-oxo acid dehydrogenases. Special focus concerns the mechanisms of function and regulation of a multienzyme structure, additional to those inherent in its component enzymes. The overall physiological process of 2-oxo acid oxidative decarboxylation and the substrate specificity determinants are considered along with side reactions of regulatory significance, such as non-oxidative reactions, formation of reactive oxygen species (ROS) and complex-bound thiyl radicals. The significance of the chemical interplay between the complex-bound dihydrolipoate intermediate, thioredoxin or thioredoxin-like proteins, and peroxiredoxins for sensing metabolic state and adjusting the catalysis accordingly, is considered. Other mechanisms involved in the short- and long-term regulation of 2-oxo acid dehydrogenase complexes are summarized in view of their relevance to physiological functions. Metabolic regulation by natural effectors of 2-oxo acid dehydrogenase complexes and their synthetic analogues is considered. The medical implications of enzymological knowledge on the complexes are also presented.
Mai multe cărți electronice de la același autor (i) / Editor
47.233 Ebooks din această categorie
