Plant-parasitic and free-living nematodes are increasingly important in relation to food security, quarantine measures, ecology (including pollution studies), and research on host-parasite interactions. Being mostly microscopic, nematodes are challenging organisms for research. Techniques for Work with Plant and Soil Nematodes introduces the basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes.
Written by an international team of experts, this book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The book covers areas that have become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes. Information on physiological assays, electron microscopy techniques and basic information on current molecular methodologies and their various applications is also included.
This book is an essential resource for students of nematology and parasitology, academic researchers, diagnostic laboratories, and quarantine and advisory service personnel. It provides a much-needed methodology standard for anyone involved in work on plant and soil nematodes.
About the author
Dr Sergei Subbotin obtained an MSc in Plant Protection in 1981 from the Moscow Agricultural Academy in Russia. In the same year, he joined the Plant Pathology Laboratory in the V. R. Williams All-Russian Fodder Research Institute, Moscow region, and later All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin (former VIGIS), Moscow, where he was awarded a Ph D in Helminthology (Nematology) in 1987. Dr Subbotin has worked as a researcher in the Center of Parasitology of the A. N. Severtsov Institute of Ecology and Evolution (Moscow, Russia), Rothamsted Experimental Station (now Rothamsted Research, Harpenden, UK), Institute for Agricultural and Fisheries Research (ILVO, Merelbeke, Belgium), Julius Ku¨hn-Institut (Mu¨nster, Germany), Department of Nematology, University of California (Riverside, USA) and as a Visiting Professor in Ghent University, Belgium. Presently, he serves as Senior Nematologist in the Plant Pest Diagnostic Center, California Department of Food and Agriculture, Sacramento, California, USA, and has affiliation positions as Scientist in the Center of Parasitology, Russia and Associate Scientist in the University of California, USA. Dr Subbotin is one of the Founders and Chief Editors of the Russian Journal of Nematology. He has published more than 190 scientific articles in peer-reviewed journals on different aspects of molecular systematics and diagnostics of nematodes, and is the author of 22 book chapters. He is co-author of the two volume Systematics of Cyst Nematodes (Nematoda: Heteroderinae) (2010) (Brill, The Netherlands), Systematics of the Sheath Nematodes of the Superfamily Hemicycliophoroidea (2014) (Brill) and Entomoparasitic Nematodes of the order Tylenchida and Aphelenchida (2019) (in Russian; KMK, Russia), and co-editor of the two volume Plant Parasitic Nematodes in Sustainable Agriculture of North America (2018) (Springer, Germany). His research studies have focused on molecular systematics, diagnostics and phylogeography of nematodes.
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