A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (h ES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of h ES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.