This book constitutes the refereed proceedings of the 7th International Conference on Theory and Practice of Natural Computing, TPNC 2017, held in Dublin, Ireland, in December 2018. The 35 full papers presented in this book, together with one invited talk, were carefully reviewed and selected from 69 submissions. The papers are organized around the following topical sections: applications of natural computing as algorithms, bioinformatics, control, cryptography, design, economics. The more theoretical contributions handle with artificial chemistry, artificial immune systems, artificial life, cellular automata, cognitive computing, cognitive engineering, cognitive robotics, collective behaviour, complex systems, computational intelligence, computational social science, computing with words, developmental systems, DNA computing, DNA nanotechnology, evolutionary algorithms, evolutionary computing, evolutionary game theory, fractal geometry, fuzzy control, fuzzy logic, fuzzy sets, fuzzy systems, genetic algorithms, genetic programming, granular computing, heuristics, intelligent agents, intelligent systems, machine intelligence, molecular programming, neural computing, neural networks, quantum communication, quantum computing, rough sets, self-assembly.