DNA replication is a key event in the cell cycle. Althoughour knowledge is far from complete and many elusiveregulatory mechanisms still remain beyondour grasp, manyenzymes and a multiplicity of biochemical mechanismsinvolved have been discovered. Recent findings in E. colihave confirmed and yet surpassed the original hypothesis of F. Jacob. In yeast and higher eucaryotes, the apparentredundancy in putative origins and initiators has made anestimation of the importance of each identified elementdifficult to access. In spite of well establishedmethodologies – which are also described in the book – theorigin identification in mammalian chromosomes is still acontroversial subject. On the other hand, considerableadvances have been made in our understanding of virus DNAreplication and this continues to deepen and broaden ourunderstanding of the controls of cellular DNA replication.
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