Pang-Chui Shaw & Hui Cao 
AUTHEN CHN MED MATER .. (2ND ED) [EPUB ebook] 
Techniques and Applications

For centuries, Chinese medicinal materials have been used for therapeutic purposes. Chinese medicinal materials are traditionally identified by their organoleptic characteristics, such as the texture or the odor. With the advancement of DNA technology, a molecular approach has become an important tool to complement organoleptic, morphological, anatomical and chemical techniques for the authentication and quality assurance of Chinese medicinal materials.

In 2002, the authors published Authentication of Chinese Medicinal Materials by DNA Technology which is the first international reference in this field. After 20 years, this new edition updates the various useful techniques and describes the new techniques developed for molecular authentication. The procedures of each DNA technique are provided in detail for ‘step-by-step’ experiments.

This book is divided into 5 parts with 18 chapters. Part I reviews the current status of molecular authentication and introduces a wide range of DNA techniques. Part II lists the experimental procedures for molecular authentication. Part III describes the DNA fingerprinting-based techniques. Part IV describes the DNA sequencing-based techniques. Finally, Part V provides an account on the recent advancement in molecular authentication, including guidelines for setting up a proper DNA laboratory and concluding remarks.

Contents:

• Introduction:
  
  
  
  • General Introduction
  
  


• General Methodology and Techniques for Molecular Authentication:
  
  
  
  • DNA Extraction for Medicinal Materials (Hoi-Yan Wu, Forrest Chung-Fai Yau & Fai-Ngor Ngan)
  
  
  • General Methodology (Mavis Hong-Yu Yik)
  
  
  • Bioinformatics for DNA Sequencing Data Analysis (Yat-Tung Lo)
  
  


• DNA Fingerprinting-based Techniques for Molecular Authentication:
  
  
  
  • Authentication of Medicinal Materials and Their Formula by Randomly Primed Polymerase Chain Reaction (Hui Cao, Ying Zhang, Kur-Ta Cheng & Pang-Chui Shaw)
  
  
  • Authentication of Medicinal Materials by Amplified Fragment Length Polymorphism (Hui Cao, Ying Zhang & Pang-Chui Shaw)
  
  
  • Authentication of Medicinal Materials by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (Hui Cao, Ying Zhang, Ping Li & Katsuko Komatsu)
  
  
  • Authentication of Medicinal Materials by Sequence Characterized Amplified Region (Grace Wing-Chiu But, Jun Wang, Wai-Yan Ha, Forrest Chung-Fai Yau, Paul Pui-Hay But & Pang-Chui Shaw)
  
  
  • Authentication of Medicinal Materials by Multiplex Polymerase Chain Reaction (Zheng-Fei Lu)
  
  


• DNA Sequencing-based Techniques for Molecular Authentication:
  
  
  
  • Identification of Medicinal Materials Using Forensically Informative Nucleotide Sequencing (Ming Li)
  
  
  • DNA Barcoding as a Standard Molecular Authentication Technique (Hui Yao, Jian-Ping Han, Xiaohui Pang, Lin-Chun Shi, Li Xiang, Xi-Wen Li, Chao Xiong, Jing-Yuan Song & Shi-Lin Chen)
  
  
  • Authentication of Medicinal Products by Adaptor Ligation-Mediated Polymerase Chain Reaction and Metabarcoding (Yat-Tung Lo, Mavis Hong-Yu Yik & Xiao Lin)
  
  
  • Super-Barcoding of Medicinal Materials Based on Complete Plastid Genomes and Nuclear Ribosomal DNA (Hyun-Seung Park, Padmanaban Mohanan & Tae-Jin Yang)
  
  


• Advanced Applications and Outlook of Molecular Authentication Techniques:
  
  
  
  • Rapid Detection of CITES-Listed Shark Species Using Loop-Mediated Isothermal Amplification (Grace Wing-Chiu But, Hoi-Yan Wu, Kwang-Tsao Shao & Pang-Chui Shaw)
  
  
  • Rapid Identification of Medicinal Materials by Lateral Flow Dipstick Diagnosis (Grace Wing-Chiu But, Ka-Lok Wong & Pang-Chui Shaw)
  
  
  • Barcoding High-Resolutions Melting Analysis (Suchada Sukrong, Kannika Thongkhao, Chayapol Tungphatthong & Thatree Phadungcharoen)
  
  
  • Setting Up a DNA Laboratory for Molecular Authentication (Ka-Lok Wong & Wai-Yan Ha)
  
  
  • Concluding Remarks
  
  

Readership: Advanced undergraduate and graduate students, researchers and practitioners in the fields of molecular authentication, testing laboratory, DNA biotechnology and Chinese medicine.

Review of the First Edition: Overall it is a useful book for anyone involved in the authentication and quality control of botanical drugs, but especially those of TCM origin. – Journal of Ethnopharmacology

Key Features:

• This book provides updates on classical DNA techniques and describes the latest techniques for molecular authentication of Chinese medicinal materials


• The procedures of each DNA technique are provided in detail for ‘step-by-step’ experiments