Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.
The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.
Power Point slides of the figures as well as other supplementary materials for instructors are available at a companion website:
www.wiley.com/go/murphy/lightmicroscopy
Зміст
Chapter 1: Fundamentals of Light Microscopy
Chapter 2: Light and Color
Chapter 3: Illuminators, Filters, and the Isolation of Specific Wavelengths
Chapter 4: Lenses and Geometrical Optics
Chapter 5: Diffraction and Interference in Image Formation
Chapter 6: Diffraction and Spatial Resolution
Chapter 7: Phase Contrast Microscopy and Darkfield Microscopy
Chapter 8: Properties of Polarized Light
Chapter 9: Polarization Microscopy
Chapter 10: Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy
Chapter 11: Fluorescence Microscopy
Chapter 12: Fluorescence Imaging of Dynamic Molecular Processes
Chapter 13: Confocal Laser Scanning Microscopy
Chapter 14: Two-Photon Excitation Fluorescence Microscopy
Chapter 15: Superresolution Imaging
Chapter 16: Imaging Living Cells With The Microscope
Chapter 17: Fundamentals of Digital Imaging
Chapter 18: Digital Imaging Processing
Appendix I
Appendix II
Appendix III
Glossary
References
Index
Про автора
DOUGLAS B. MURPHY supervises core facilities in
microscopy and histology at the new HHMI Janelia Farm Research
Campus in Ashburn, Virginia. An Adjunct Professor of Cell Biology
at Johns Hopkins School of Medicine in Baltimore, Maryland, Dr.
Murphy helped establish the School of Medicine Microscope Facility
there, which he supervised until 2006.
MICHAEL W. DAVIDSON is an assistant scholar/scientist
affiliated with the National High Magnetic Field Laboratory and the
Department of Biological Science at Florida State University where
he is involved in developing educational websites. His digital
images and photomicrographs have graced the covers of over 2, 000
publications.
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