This text is a review of molecular immunohematology (MI). It draws from analyses and case studies around the world and details many techniques used in many labs. It is aimed at anyone interested in how MI is changing blood bank and transfusion medicine.
Зміст
An overview of the Classical Serological methods: limitations and benefits of serology and DNA testing.- Introduction to Molecular Typing.- The Bead Chip System: A Flexible Array Format for Complex Nucleic Acid and Protein Analysis.- Implementation of HEA at Blood Centers: Prescreening, Rare Donors, Inventory Management.- Implementation of HEA Bead Chip System at Medical Centers: Providing Extended Matched Units and Eliminating Complex Workups for Patients.- Human Platelet Antigen Genotyping and Diagnosis of Antiplatelet Alloimmunization.- Blood group genotyping by high-throughput DNA-analysis: Application to the Panel National de Référence du CNRGS.- Implementation and Assessment of High-Throughput Donor Typing at the Milan, Italy Immunohematology Reference Laboratory.- Implementation of the Bio Array Erythrocyte Antigen (HEA) genotyping System Bead Chip™ HEA: The Red Cross of Spain Experience.- Looking Beyond HEA: Matching SCD Patients for RH Variants Marion E Reid*, Christine Halter Hipsky.- Identification of Altered RHD and RHCE Alleles: A Comparison of Manual and Automated Molecular Methods.- Bayesian Classification Algorithms for Automated Allele Assignment.
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