Juan M. Ruso & Ángel Piñeiro 
Proteins in Solution and at Interfaces [PDF ebook] 
Methods and Applications in Biotechnology and Materials Science

Explores new applications emerging from our latest
understanding of proteins in solution and at interfaces

Proteins in solution and at interfaces increasingly serve as the
starting point for exciting new applications, from biomimetic
materials to nanoparticle patterning. This book surveys the state
of the science in the field, offering investigators a current
understanding of the characteristics of proteins in solution and at
interfaces as well as the techniques used to study these
characteristics. Moreover, the authors explore many of the new and
emerging applications that have resulted from the most recent
studies. Topics include protein and protein aggregate structure;
computational and experimental techniques to study protein
structure, aggregation, and adsorption; proteins in non-standard
conditions; and applications in biotechnology.

Proteins in Solution and at Interfaces is divided into
two parts:

* Part One introduces concepts as well as theoretical and
experimental techniques that are used to study protein systems,
including X-ray crystallography, nuclear magnetic resonance, small
angle scattering, and spectroscopic methods

* Part Two examines current and emerging applications,
including nanomaterials, natural fibrous proteins, and biomolecular
thermodynamics

The book’s twenty-three chapters have been contributed by
leading experts in the field. These contributions are based on a
thorough review of the latest peer-reviewed findings as well as the
authors’ own research experience. Chapters begin with a discussion
of core concepts and then gradually build in complexity, concluding
with a forecast of future developments.

Readers will not only gain a current understanding of proteins
in solution and at interfaces, but also will discover how
theoretical and technical developments in the field can be
translated into new applications in material design, genetic
engineering, personalized medicine, drug delivery, biosensors, and
biotechnology.

Зміст

PREFACE ix

CONTRIBUTORS xiii

PART I

1 X-Ray Crystallography of Biological Macromolecules:
Fundamentals and Applications 3

Antonio L. Llamas-Saiz and Mark J. van Raaij

2 Nuclear Magnetic Resonance Methods for Studying Soluble,
Fibrous, and Membrane-Embedded Proteins 23

Victoria A. Higman

3 Small-Angle X-Ray Scattering Applied to Proteins in Solution
49

Leandro Ramos Souza Barbosa, Francesco Spinozzi, Paolo Mariani,
and Rosangela Itri

4 Analyzing the Solution State of Protein Structure,
Interactions, and Ligands by Spectroscopic Methods 73

Veronica I. Dodero and Paula V. Messina

5 Resolving Membrane-Bound Protein Orientation and Conformation
by Neutron Reflectivity 99

Hirsh Nanda

6 Investigating Protein Interactions at Solid Surfaces–In
Situ, Nonlabeling Techniques 113

Olof Svensson, Javier Sotres, and Alejandro Barrantes

7 Calorimetric Methods to Characterize the Forces Driving
Macromolecular Association and Folding Processes 139

Conceic¸ ~ao A.S.A. Minetti, Peter L. Privalov, and
David P. Remeta

8 Virtual Ligand Screening Against Comparative Models of
Proteins 179

Hao Fan

9 Atomistic and Coarse-Grained Molecular Dynamics Simulations of
Membrane Proteins 193

Thomas J. Piggot, Peter J. Bond, and Syma Khalid

PART II

10 Preparation of Nanomaterials Based on Peptides and Proteins
209

Yujing Sun and Zhuang Li

11 Natural Fibrous Proteins: Structural Analysis, Assembly, and
Applications 219

Mark J. van Raaij and Anna Mitraki

12 Amyloid-Like Fibrils: Origin, Structure, Properties, and
Potential Technological Applications 233

Pablo Taboada, Silvia Barbosa, Josue Juarez, Manuel-Alatorre
Meda, and Vyctor Mosquera

13 Proteins and Peptides in Biomimetic Polymeric Membranes
283

Alfredo Gonzalez-Perez

14 Study of Proteins and Peptides at Interfaces By Molecular
Dynamics Simulation Techniques 291

David Poger and Alan E. Mark

15 A Single-Molecule Approach to Explore the Role of the Solvent
Environment in Protein Folding 315

Katarzyna Tych and Lorna Dougan

16 Enhanced Functionality of Peroxidases By Its Immobilization
at The Solid-Liquid Interface of Mesoporous Materials and
Nanoparticles 335

Jose Campos-Teran, Iker Inarritu, Jorge Aburto, and Eduardo
Torres

17 Superactivity of Enzymes in Supramolecular Hydrogels
353

Ye Zhang and Bing Xu

18 Surfactant Proteins and Natural Biofoams 365

Malcolm W. Kennedy and Alan Cooper

19 Promiscuous Enzymes 379

Luis F. Olguin

20 Thermodynamics and Kinetics of Mixed Protein/Surfactant
Adsorption Layers at Liquid Interfaces 389

Reinhard Miller, E.V. Aksenenko, V.S. Alahverdjieva, V.B.
Fainerman, C.S. Kotsmar, J. Kragel, M.E. Leser, J.
Maldonado-Valderrama, V. Pradines, C. Stefaniu, A. Stocco, and R.
Wustneck

21 Application of Force Spectroscopy Methods to the Study of
Biomaterials 429

Chuan Xu and Erika F. Merschrod S.

22 Protein Gel Rheology 437

Katie Weigandt and Danilo Pozzo

23 Exploring Biomolecular Thermodynamics in Aqueous and
Nonaqueous Environments using Time-Resolved Photothermal Methods
449

Randy W. Larsen, Carissa M. Vetromile, William A. Maza, Khoa
Pham, Jaroslava Miksovska

INDEX 473

Про автора

JUAN M. RUSO is currently Associate Professor in the
Department of Applied Physics at the University of Santiago de
Compostela, Spain. He has contributed to more than 100 publications
on a broad range of physical, chemical, and biophysics studies. His
research interests include protein ligand interactions, thermal
stabilization of protein, Phase behavior, and self-assembly
processes in soft matter systems, biocompatible materials design,
and nanocarrier design for targeted drug delivery.

ÁNGEL PIÑEIRO is an IPP Research Fellow at the
Department of Applied Physics of the University of Santiago de
Compostela, Spain. His current research interests include the
design and characterization of self-assembled systems as well as
the study of macromolecules in solution, embedded in membranes or
at interfaces. His work is mainly based on computational methods
including multiscale molecular dynamics simulations and the
development of software for the analysis of different experimental
properties.