The opening chapter of Advances in Medicine and Biology. Volume 144 summarizes the generation of amniotic fluid, role and its clinical significance of protecting the fetus from external trauma. Next, the role of in silico and in vitro methods are used for the prediction of cytotoxicity. Additionally the authors discuss the principles of aptamer selection and in vitro applications for bench scientists in molecular imaging, including aptahistochemistry, tissue imaging, enzyme-linked aptamer assay, aptablot, aptamer-mediated flow cytometry and mass cytometry, and super-resolution microscopy. In order for natural rubber latex nanoparticles to be employed in tissue engineering, they need to have designed biological interaction with cells as well as favorable mechanical properties. Cells can delicately sense and respond to external nanoscale features in intricate living systems. With this in mind, chapter four demonstrates an in-depth understanding of how cell differentiation may bring a new perspective to tissue engineering including new bone regeneration and cartilage therapy. In the penultimate chapter, a study of the relationship between the adipokine signaling dysregulation and the androgen status is proposed as necessary to develop new adipokines-based approaches to restore the steroidogenesis and spermatogenesis in male in the metabolic and endocrine diseases. In recent stress research, coping flexibility, which is defined as an individual’s ability to modify their coping strategies effectively according to the demands of different stressful situations, has received considerable attention. The author of the closing chapter presents two cross-sectional studies in order to examine the relationship between insomnia and coping flexibility for daily stress.
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