This edited book is focusing on the novel and innovative procedures in tissue culture for large scale production of plantation and horticulture crops. It is bringing out a comprehensive collection of information on commercial scale tissue culture with the objective of producing high quality, disease-free and uniform planting material. Developing low cost commercial tissue culture can be one of the best possible way to attain the goal of sustainable agriculture. Tissue culture provides a means for rapid clonal propagation of desired cultivars, and a mechanism for somatic hybridization and in vitro selection of novel genotypes. Application of plant tissue culture technology in horticulture and plantation crops provides an efficient method to improve the quality and nutrition of the crops.
This book includes a description of highly efficient, low cost in vitro regeneration protocols of important plantation and horticulture crops with a detailed guideline to establish a commercialplant tissue culture facility including certification, packaging and transportation of plantlets. The book discusses somatic embryogenesis, virus elimination, genetic transformation, protoplast fusion, haploid production, coculture of endophytic fungi, effects of light and ionizing radiation as well as the application of bioreactors.
This book is useful for a wide range of readers such as, academicians, students, research scientists, horticulturists, agriculturists, industrial entrepreneurs, and agro-industry employees.
Зміст
Chapter 1. Improved sterilization techniques for successful in vitro micropropagation.- Chapter 2. Selection and preparation of explants for the clonal propagation of horticultural plants in plant factory systems.- Chapter 3. Use of alternative components in cost-effective media for mass production of clonal plants.- Chapter 4. Novel plant growth regulators in in vitro establishment of horticulture and plantation crops.- Chapter 5. Potential role and utilization of Piriformospora indica: fungal endophytes in commercial plant tissue culture.- Chapter 6. Application of biostimulants in establishing and acclimatizing in vitro-raised plants.- Chapter 7. Nanomaterials and plant tissue culture: Developmental path and contradictory facts.- Chapter 8. Somatic embryogenesis in cashew (Anacardium occidentale L.).- Chapter 9. Somatic embryogenesis and plant regeneration in horticultural crops.- Chapter 10. Prospect and commercial production of economically important plant mulberry (Morus sp.) towards the upliftment of rural economy.- Chapter 11. Assessing the genetic stability of in vitro raised plants.- Chapter 12. Improvement of plant survival and expediting acclimatization process.- Chapter 13. Evaluation of genetic stability of in vitro raised orchids using molecular based markers.- Chapter 14. Recent in-vitro propagation advances in Genus Paphiopedilum – Lady Slipper Orchids.
Про автора
Dr Shubhpriya Gupta, completed her Ph.D. in Botany from G.B. Pant University of Agriculture and Technology, Pantnagar, India. Currently, she is working as a Post Doctoral Researcher at Palacký University Olomouc, Czech Republic, and as a visiting Scientist at the University of Kwa Zulu-Natal, Pietermaritzburg, South Africa. She is the Founder and Director of Gustaden Manuscript Services, India. Her research field includes plant hormones, biostimulants, plant tissue culture, seed physiology, analytical chemistry, and biotechnology. She is the author/coauthor of 20 peer-reviewed articles, which have been published in ISI-rated journals. She is the review board editor of the South African Journal of Botany. She has received an Y1 (Young researcher) rating from the National Research Foundation (NRF), South Africa in January 2022.
Dr Preeti Chaturvedi, is Professor and Head, Department of Biological Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar. She did her M.Sc. and Ph.D. in Botany from Kurukshetra University, Kurukshetra, India. Her research interests include in vitro morphogenesis, conservation biology, bryophyte diversity, and bioprospecting. She has successfully developed micropropagation protocols for several high value medicinal plants viz., Aconitum balfourii, Picrorhiza kurroa, Centella asiatica, Polygonatum verticillatum, and Rheum emodii. She has 65 research publications to her credit in national and international journals of repute. She has completed four research projects on micropropagation of medicinal herbs including one on bioprospecting of bryophytes.