This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of micro RNAs (mi RNAs) in plants and animals. After overviews of how best to detect, identify, and validate micro RNAs, the book continues by exploring state-of-the-art protocols for micro RNA detection, approaches to profile the expression level of micro RNAs, spatial expression analysis, describe in silico analysis of micro RNAs and their targets, as well as protocols for functional analysis of micro RNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Micro RNA Detection and Target Identification: Methods and Protocols, Second Edition aimsto ensure successful results in the further study of this vital field.