This second edition provides a broad spectrum of methods used in long non-coding RNAs (lnc RNA) research, ranging from computational annotation of lnc RNA genes to molecular and cellular analyses of the function of individual lnc RNA. Chapters guide readers through studies used to circular RNAs, RNA splicing, and findings on lnc RNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Long Non-Coding RNAs: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
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