This book is a practical guidebook in biochemistry, for medical as well as life sciences’ students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations, The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
Table of Content
Common clinical laboratory hazards and waste disposal.- Blood collection and preservation.- Quality Control in laboratory.- Automation in clinical laboratory.- Photometry-Colorimeter and Spectrophotometer.- Preparation of general laboratory solutions and buffers.- Examination of urine for normal constituents.- To perform qualitative tests for urinary proteins.- To determine the quantity of proteins in urine sample using Biuret reaction.- To estimate the amount of total protein and albumin in serum and to find A/G ratio.- To perform qualitative test for reducing substances in urine.- Quantitative analysis of reducing sugars in urine.- Estimation of blood glucose levels by Glucose Oxidase method.- Estimation of blood glucose levels by Folin and Wu method.- To perform Glucose Tolerance Test.- Estimation of urea in serum and urine.- To determine urea clearance.- To estimate creatinine level in serum and urine by Jaffe’s reaction.- To determine creatinine clearance.- To determine the uric acid concentration in serum and urine.- Estimation of total calcium in serum and urine.- Estimation of inorganic phosphorus in serum and urine.- To estimate the amount of total cholesterol in serum.- To estimate total and direct bilirubin in serum.- To determine Alanine and Aspartate Transaminases activity in serum.- To estimate the activity of alkaline phosphatase in serum.- To estimate the activity of acid phosphatase in serum.- To determine serum and urinary amylase activity.- To estimate the activity of lipase in serum.- Qualitative analysis of ketone bodies in urine.- Qualitative test for bile pigments and urobilinogen in urine.- Determination of total lactate dehydrogenase activity in serum.- To measure the activity of Creatine kinase-MB and Total Creatine kinase in serum.- Analysis of Cerebrospinal Fluid for proteins and sugars.- To analyze lipid profile from given serum sample.- To determine sodium and potassium in serum by using Flame photometry.- To perform Radioimmunoassay.-To perform Enzyme linked Immunosorbant assay.- Some important case studies.
About the author
Prof. Kiran Dip Gill obtained her Ph.D. degree in Biochemistry from Punjab Agriculture University, Ludhiana, India in 1974. She was a postdoctoral fellow at University of Gottingen, Germany in 1977-1978. In 1981, she joined department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India. She has taught Biochemistry to post-graduate students for almost 35 years. She was also in-charge of clinical biochemistry lab, where biochemical investigations are done routinely. She has authored four chapters in books and published more than 125 research papers in her career. She is recipient of several awards for her contribution in research work.
Dr. Vijay Kumar obtained his Ph.D. degree in Biochemistry under the guidance of Prof. Kiran Dip Gill from Post Graduate Institute of Medical Education and Research, Chandigarh in 2009. In 2010, he joined Maharshi Dayanand University, Rohtak. Since then, he is actively involved in teaching Biochemistry to post-graduate students. He has published more than 20 research papers and three review articles in reputed journals.
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